Skip to main content
PMC home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1994 Jan;53(1):35–38. doi: 10.1136/ard.53.1.35

Zonal distribution of chondroitin-4-sulphate/dermatan sulphate and chondroitin-6-sulphate in normal and diseased human synovium.

J G Worrall 1, L S Wilkinson 1, M T Bayliss 1, J C Edwards 1
PMCID: PMC1005240  PMID: 8311553

Abstract

OBJECTIVES--Chondroitin sulphate is the major sulphated glycosaminoglycan present in the extracellular matrix of soft connective tissues and the aim of this study was to investigate the distribution of chondroitin sulphate species in normal and diseased synovium. METHODS--Distribution of chondroitin-4-sulphate/dermatan sulphate (Ch4S/DS) and chondroitin-6-sulphate in normal (n = 6), osteoarthritic (n = 4) and rheumatoid (n = 10) synovium was determined using an immunoperoxidase technique and specific monoclonal antibodies to chondroitinase ABC-digested preparations. RESULTS--Ch4S/DS was expressed throughout the interstitium of all tissues and was also present on blood vessels in rheumatoid samples only. Ch6S was expressed in the lining layer of normal synovium but was absent from this site in osteoarthritic and rheumatoid tissues. Ch6S was also present on all blood vessels in all tissues. CONCLUSIONS--The distinct zonal distributions of Ch4S/DS and Ch6S and their alteration in disease suggest these molecules have different and specific functions in normal and diseased synovium.

Full text

PDF
35

Images in this article

37Image
on p.37
37Image
on p.37
37Image
on p.37

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aruffo A., Stamenkovic I., Melnick M., Underhill C. B., Seed B. CD44 is the principal cell surface receptor for hyaluronate. Cell. 1990 Jun 29;61(7):1303–1313. doi: 10.1016/0092-8674(90)90694-a. [DOI] [PubMed] [Google Scholar]
  2. Barker S. A., Hawkins C. F., Hewins M. Mucopolysaccharides in synovial fluid detection of chondroitin sulphate. Ann Rheum Dis. 1966 May;25(3):209–213. doi: 10.1136/ard.25.3.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bertolotto A., Palmucci L., Mongini T., Schiffer D., Caterson B. Chondroitin, chondroitin 6-sulphate, chondroitin 4-sulphate and dermatan sulphate proteoglycans in normal and pathological human muscle. J Neurol Sci. 1987 Nov;81(2-3):247–259. doi: 10.1016/0022-510x(87)90100-6. [DOI] [PubMed] [Google Scholar]
  4. Bröker B. M., Edwards J. C., Fanger M. W., Lydyard P. M. The prevalence and distribution of macrophages bearing Fc gamma R I, Fc gamma R II, and Fc gamma R III in synovium. Scand J Rheumatol. 1990;19(2):123–135. doi: 10.3109/03009749009102116. [DOI] [PubMed] [Google Scholar]
  5. Caterson B., Christner J. E., Baker J. R., Couchman J. R. Production and characterization of monoclonal antibodies directed against connective tissue proteoglycans. Fed Proc. 1985 Feb;44(2):386–393. [PubMed] [Google Scholar]
  6. Caterson B., Christner J. E., Baker J. R. Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate. Monoclonal antibodies to cartilage proteoglycan. J Biol Chem. 1983 Jul 25;258(14):8848–8854. [PubMed] [Google Scholar]
  7. Caterson B., Mahmoodian F., Sorrell J. M., Hardingham T. E., Bayliss M. T., Carney S. L., Ratcliffe A., Muir H. Modulation of native chondroitin sulphate structure in tissue development and in disease. J Cell Sci. 1990 Nov;97(Pt 3):411–417. doi: 10.1242/jcs.97.3.411. [DOI] [PubMed] [Google Scholar]
  8. Couchman J. R., Caterson B., Christner J. E., Baker J. R. Mapping by monoclonal antibody detection of glycosaminoglycans in connective tissues. Nature. 1984 Feb 16;307(5952):650–652. doi: 10.1038/307650a0. [DOI] [PubMed] [Google Scholar]
  9. Folkman J., Klagsbrun M., Sasse J., Wadzinski M., Ingber D., Vlodavsky I. A heparin-binding angiogenic protein--basic fibroblast growth factor--is stored within basement membrane. Am J Pathol. 1988 Feb;130(2):393–400. [PMC free article] [PubMed] [Google Scholar]
  10. Freemont A. J., Jones C. J., Bromley M., Andrews P. Changes in vascular endothelium related to lymphocyte collections in diseased synovia. Arthritis Rheum. 1983 Dec;26(12):1427–1433. doi: 10.1002/art.1780261203. [DOI] [PubMed] [Google Scholar]
  11. Gallagher J. T. The extended family of proteoglycans: social residents of the pericellular zone. Curr Opin Cell Biol. 1989 Dec;1(6):1201–1218. doi: 10.1016/s0955-0674(89)80072-9. [DOI] [PubMed] [Google Scholar]
  12. Kanwar Y. S., Hascall V. C., Farquhar M. G. Partial characterization of newly synthesized proteoglycans isolated from the glomerular basement membrane. J Cell Biol. 1981 Aug;90(2):527–532. doi: 10.1083/jcb.90.2.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Levick J. R., McDonald J. N. Microfibrillar meshwork of the synovial lining and associated broad banded collagen: a clue to identity. Ann Rheum Dis. 1990 Jan;49(1):31–36. doi: 10.1136/ard.49.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Obrink B., Laurent T. C., Carlsson B. The binding of chondroitin sulphate to collagen. FEBS Lett. 1975 Aug 1;56(1):166–169. doi: 10.1016/0014-5793(75)80133-5. [DOI] [PubMed] [Google Scholar]
  15. Okada Y., Nagase H., Harris E. D., Jr A metalloproteinase from human rheumatoid synovial fibroblasts that digests connective tissue matrix components. Purification and characterization. J Biol Chem. 1986 Oct 25;261(30):14245–14255. [PubMed] [Google Scholar]
  16. Parthasarathy N., Spiro R. G. Characterization of the glycosaminoglycan component of the renal glomerular basement membrane and its relationship to the peptide portion. J Biol Chem. 1981 Jan 10;256(1):507–513. [PubMed] [Google Scholar]
  17. Pollock L. E., Lalor P., Revell P. A. Type IV collagen and laminin in the synovial intimal layer: an immunohistochemical study. Rheumatol Int. 1990;9(6):277–280. doi: 10.1007/BF00541324. [DOI] [PubMed] [Google Scholar]
  18. Sorrell J. M., Mahmoodian F., Schafer I. A., Davis B., Caterson B. Identification of monoclonal antibodies that recognize novel epitopes in native chondroitin/dermatan sulfate glycosaminoglycan chains: their use in mapping functionally distinct domains of human skin. J Histochem Cytochem. 1990 Mar;38(3):393–402. doi: 10.1177/38.3.1689338. [DOI] [PubMed] [Google Scholar]
  19. Worrall J. G., Bayliss M. T., Edwards J. C. Morphological localization of hyaluronan in normal and diseased synovium. J Rheumatol. 1991 Oct;18(10):1466–1472. [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES