Abstract
OBJECTIVES--To investigate the prevalence and characteristics of antibodies to endothelial cells (aEC) from large vessel and from microvasculature in a group of patients with Behçet's disease (BD) to determine the relationship of these antibodies with clinical and laboratory features of the disease. METHODS--Thirty patients with BD were prospectively and consecutively studied. The aEC were determined by enzyme-linked immunosorbent assay (ELISA) using endothelial cells derived from human umbilical vein (large vessel) as well as from retroperitoneal adipose tissue (microvasculature). RESULTS--Fifteen patients (50%) had aEC, either directed to large vessel [8(26%) patients] or microvascular [13(43%) patients] endothelial cells. The percentage of active patients was significantly higher in the aEC-positive group [12(80%) patients] compared with the aEC-negative group [5(33%) patients] (p < 0.05). CONCLUSIONS--Patients with BD have a high prevalence of aEC when microvascular endothelial cells are used in the assay. These antibodies seem to be a marker of disease activity in this condition, previously considered as negative for autoantibodies.
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- Aydìntug A. O., Tokgöz G., D'Cruz D. P., Gürler A., Cervera R., Düzgün N., Atmaca L. S., Khamashta M. A., Hughes G. R. Antibodies to endothelial cells in patients with Behçet's disease. Clin Immunol Immunopathol. 1993 May;67(2):157–162. doi: 10.1006/clin.1993.1059. [DOI] [PubMed] [Google Scholar]
- Cervera R., Khamashta M. A., Font J., Ramírez G., D'Cruz D., Montalbán J., López-Soto A., Asherson R. A., Ingelmo M., Hughes G. R. Antiendothelial cell antibodies in patients with the antiphospholipid syndrome. Autoimmunity. 1991;11(1):1–6. doi: 10.3109/08916939108994701. [DOI] [PubMed] [Google Scholar]
- D'Cruz D. P., Houssiau F. A., Ramirez G., Baguley E., McCutcheon J., Vianna J., Haga H. J., Swana G. T., Khamashta M. A., Taylor J. C. Antibodies to endothelial cells in systemic lupus erythematosus: a potential marker for nephritis and vasculitis. Clin Exp Immunol. 1991 Aug;85(2):254–261. doi: 10.1111/j.1365-2249.1991.tb05714.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferraro G., Meroni P. L., Tincani A., Sinico A., Barcellini W., Radice A., Gregorini G., Froldi M., Borghi M. O., Balestrieri G. Anti-endothelial cell antibodies in patients with Wegener's granulomatosis and micropolyarteritis. Clin Exp Immunol. 1990 Jan;79(1):47–53. doi: 10.1111/j.1365-2249.1990.tb05125.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frampton G., Jayne D. R., Perry G. J., Lockwood C. M., Cameron J. S. Autoantibodies to endothelial cells and neutrophil cytoplasmic antigens in systemic vasculitis. Clin Exp Immunol. 1990 Nov;82(2):227–232. doi: 10.1111/j.1365-2249.1990.tb05431.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heurkens A. H., Hiemstra P. S., Lafeber G. J., Daha M. R., Breedveld F. C. Anti-endothelial cell antibodies in patients with rheumatoid arthritis complicated by vasculitis. Clin Exp Immunol. 1989 Oct;78(1):7–12. [PMC free article] [PubMed] [Google Scholar]
- Pivetti-Pezzi P., Priori R., Catarinelli G., Meroni P. L., Federici A. B., Abdulaziz M., Falco M., Valesini G. Markers of vascular injury in Behçet's disease associated with retinal vasculitis. Ann Ophthalmol. 1992 Nov;24(11):411–414. [PubMed] [Google Scholar]
- Rosenbaum J., Pottinger B. E., Woo P., Black C. M., Loizou S., Byron M. A., Pearson J. D. Measurement and characterisation of circulating anti-endothelial cell IgG in connective tissue diseases. Clin Exp Immunol. 1988 Jun;72(3):450–456. [PMC free article] [PubMed] [Google Scholar]