Table 1.
Ka [x 109 M−1]a | |
---|---|
HlyU reduced | 0.40 ± 0.08 (n = 4) |
HlyU, GSSH treated | 0.009 ± 0.005 (n = 2) |
HlyU, H2O2 treated | 0.19 ± 0.03 (n = 3) |
Errors correspond to standard deviations of the mean for n number of experiments carried out under the same conditions: 25 mM HEPES, pH = 7.0, 100 mM NaCl, 1 mM EDTA, 25°C. The treatment corresponds to a 5-fold addition relative to the protein subunit concentration.