Table 2.
The pros and cons of screening methods for herpesvirus mutants.
| Screening Methods | Feasibility of Continuous Editing | Applicable E. coli Strain Type | Selection Efficiency |
Auxiliary Proteins | Feasibility of Scarless Editing | Duration of Each Editing Cycle |
|---|---|---|---|---|---|---|
| Single selection | Difficult | All | >95% | None | No | 3 Days |
| combination site-specific recombinase recognition motif | Difficult | All | Approaching 100% | FLP/Cre | No | 7 Days |
| Combination I-sceI endonuclease recognition site | Feasible | All | Negtive: 1–15% | I-sceI | Yes | 7 Days |
| Positive and negative (dual) selection cassettes | Feasible |
rpsL; StrR galK; ΔgalK |
Negative: rpsL-7.8% galK-16% |
None | Yes |
rpsL-Kana: 7 Days galK: 12 Days galK-Kana: 9 Days |