Table 1.

Genome walking primers used to verify the reconstructed viral genome.

Primer Number	Sequence 5′-3′		Position (nt)	Expected Product Size (bp)
	Forward	Reverse
1	AGGTGGAGAGAATGTGCTGC	CGCGTGTATGGTAGAAGGGG	185–981	796
2	GCGGCTACCTGCTTACAGAA	CTACCTGAATGTGGGTCCCG	909–1685	786
3	TCAGCATCAGGACCAGGACA	TGCCAATTTGCTCTCCTCAGT	1596–2346	750
4	CTCCGCCCTGTTCTGGTTTA	TGGTCTCAGCTAGGGGCATA	2163–2768	605
5	GGACCACCAAACACAGGCAA	GGTTGTTTGCCACGTCTACA	2647–2939	292
6	ATGCAACCATACCTGCCTGG	TTAAGCGTGTCCTTAGGCGG	2786–3442	656
7	CCAATGCCAAAACTGCCATCTT	TATCTCGATGCGAAGGAGAAGC	3309–4101	792
8	GCCCAGGGCTACTATTGCATC	TAGGTAGAGGAAGGAGCTGACC	3948–4648	700
9	GTGCATTATGGGGCAGGACT	GGGTCGGAGAGGAAGTTTGG	4545–5301	756
10	TCCGCCTCTGACCATGTGTT	CCGGCATCGATTCAACAACC	5089–5906	817
11	TGTGTCCGTCCTGGACTTTG	AAGCGTGTCTTCCTCCAGTG	5748–6496	748
12 *	GTGTCACAGGTAACCCCCTG	AAGCGTGTCTTCCTCCAGTG	6321–6496	175
13	CAGGTGCCAAGGATGACAGG	GGCATCGGTTGTATGGAGGT	6387–7154	767
14	TGCGACATGTGGAGGAGTTT	GCATTGTTTCGGAAGCCCAG	7056–260	755
Real Time probe	TGCTGGTGGGTTGCAAGCCC		6444–6464

* Primer pair used in the Real Time assay.