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. 2023 Feb 11;42(14):1072–1087. doi: 10.1038/s41388-023-02619-4

Fig. 5. NEDD4 promotes tumor growth of IGF1R-dependent GC cells in vivo.

Fig. 5

A Representative images of tumors in nude mice after the injection of BGC803 cells stably expressing Dox-inducible NEDD4-shRNA and their controls (n = 5). B, C Quantification of tumor weights (B) and growth curves (C) of xenograft tumors in nude mice. D Representative images of tumors in nude mice after the injection of BGC803 cells transfected with NEDD4-expressing plasmids and negative control plasmids (n = 5). E, F Quantification of tumor weights (E) and growth curves (F) of xenograft tumors in nude mice. G, left Representative images of the hematoxylin and eosin (H&E) staining, Ki67, NEDD4, p-Akt s473, and PTEN IHC staining in tumor samples. G, right The percentages of positive cells were calculated by ImageJ IHC Profiler. ***p < 0.001, **p < 0.01, *p < 0.05, ns p > 0.05.