Skip to main content
. 2023 Mar 2;26(4):106314. doi: 10.1016/j.isci.2023.106314

Figure 3.

Figure 3

Constitutive PGC-1β expression supports the expression of terminal differentiation genes

(A) Gene expression in normal human epidermal keratinocytes (NHEK) transfected with control siRNA or an siRNA pool against PGC-1β for 3 days. Data are shown as mean + SEM from 3 independent experiments. ∗p < 0.05, paired Student’s t test.

(B) Correlation analysis between PPARGC1B and various gene transcripts in keratinocyte HaCaT cells transfected with 6 combinations of non-targeting, individual or pooled PGC-1β siRNA duplexes to achieve various transcript levels. Gene expression was assessed 3 days post-transfection. PPARGC1B transcript levels positively correlate with a gene set (I) of known PGC-1 metabolic targets (CS, FAS) and terminal differentiation markers (TGM1, IVL) and negatively correlates with a gene set (II) of aging-associated genes (C3, SGK1, IFIT1, and B2M).

(C) Detailed correlation analyses from B. S: Spearman r, P: Pearson r, with ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns: non-significant. Data shown in B-C are from 3 independent experiments.