Figure 5.

PGC-1s knockdown modifies reconstructed human epidermis

(A) Schematic depicting PGC-1s double knockdown strategy.

(B) Hematoxylin and eosin staining of typical reconstructed human epidermis (RHE) constructs stably expressing shRNA constructs. RHE were analyzed at day 14. This experimental set is representative of 5 independent experiments.

(C) Box-and-whisker plot of epidermal thickness measured by optical coherence tomography. Data from 15 RHE constructs per condition and obtained from 5 independent experiments are shown. ∗p < 0.05, unpaired Student’s t test.

(D) Hematoxylin and eosin staining of RHE constructs prepared with shRNA control and shRNA-PGC-1s stably expressing cells treated with DMSO (vehicle) or retinol (10 μM) from day 10 to day 19. This experimental set was analyzed at day 14, and 2 representative independent experiments are shown.

(E) Epidermal thickness measured by optical coherence tomography of RHE constructs described in D. RHE constructs were assessed on days 12, 14, 17, and 19. Data are shown as mean ± SEM from 2 to 6 independent RHE per time point.

(F) Fold change in epidermal thickness upon DMSO or retinol treatment for samples shown in E (day 17). Data are shown as mean + SEM, n = 3 independent samples per treatment group, ∗p < 0.05, two-way ANOVA, Tukey post-test.