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. 1977 Jun;36(3):209–215. doi: 10.1136/ard.36.3.209

Localization of gold in synovial membrane of rheumatoid arthritis treated with sodium aurothiomalate. Studies by electron microscope and electron probe x-ray microanalysis.

H Nakamura, M Igarashi
PMCID: PMC1006667  PMID: 406861

Abstract

The localization of gold in the synovial membrane of rheumatoid arthritis patients treated with sodium aurothiomalate was examined and quantitative analysis of epon-embedded sections was carried out with a wavelength dispersive x-ray microanalyser. Gold was only detected in the lysosomes of synovial lining type A cells and subsynovial mononuclear cells in the form of filamentous deposits and highly electron-dense granules, the latter being few in number. The concentration of gold within the lysosomes containing the characteristic deposits and granules was equivalent to that in epon-embedded standard specimens of freeze-dried albumin in which 2-0-87-4 mg/ml of gold was included. In addition, sulphur was detected in the lysosomes containing the filamentous deposits, but the S/Au x-ray signal ratio was not equal to that detected in sodium aurothiomalate. The significance of the coexistence of gold with sulphur in lysosomes is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adam M., Bartl P., Deyl Z., Rosmus J. Uptake of gold by collagen in gold therapy. Ann Rheum Dis. 1965 Jul;24(4):378–381. doi: 10.1136/ard.24.4.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BALL J., CHAPMAN J. A., MUIRDEN K. D. THE UPTAKE OF IRON IN RABBIT SYNOVIAL TISSUE FOLLOWING INTRA-ARTICULAR INJECTION OF IRON DEXTRAN. A LIGHT AND ELECTRON MICROSCOPE STUDY. J Cell Biol. 1964 Aug;22:351–364. doi: 10.1083/jcb.22.2.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BARLAND P., NOVIKOFF A. B., HAMERMAN D. FINE STRUCTURE AND CYTOCHEMISTRY OF THE RHEUMATOID SYNOVIAL MEMBRANE, WITH SPECIAL REFERENCE TO LYSOSOMES. Am J Pathol. 1964 May;44:853–866. [PMC free article] [PubMed] [Google Scholar]
  4. CAYGILL J. C., JEVONS F. R. PRESENCE OF GLUCOSAMINIDASE ACTIVITY IN HUMAN SYNOVIAL FLUID AND ITS INHIBITION BY GOLD COMPOUNDS. Clin Chim Acta. 1965 Mar;11:233–237. doi: 10.1016/0009-8981(65)90068-9. [DOI] [PubMed] [Google Scholar]
  5. Doré J. L., Vernon-Roberts B. A method for the selective demonstration of gold in tissue sections. Med Lab Sci. 1976 Jul;33(3):209–213. [PubMed] [Google Scholar]
  6. Ennis R. S., Granda J. L., Posner A. S. Effect of gold salts and other drugs on the release and activity of lysosomal hydrolases. Arthritis Rheum. 1968 Dec;11(6):756–764. doi: 10.1002/art.1780110605. [DOI] [PubMed] [Google Scholar]
  7. Ghadially F. N., Oryschak A. F., Mitchell D. M. Ultrastructural changes produced in rheumatoid synovial membrane by chrysotherapy. Ann Rheum Dis. 1976 Feb;35(1):67–72. doi: 10.1136/ard.35.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ghadially F. N., Roy S. Ultrastructure of synovial membrane in rheumatoid arthritis. Ann Rheum Dis. 1967 Sep;26(5):426–443. doi: 10.1136/ard.26.5.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grahame R., Billings R., Laurence M., Marks V., Wood P. J. Tissue gold levels after chrysotherapy. Ann Rheum Dis. 1974 Nov;33(6):536–539. doi: 10.1136/ard.33.6.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HIROHATA K., KOBAYASHI I. FINE STRUCTURES OF THE SYNOVIAL TISSUES IN RHEUMATOID ARTHRITIS. Kobe J Med Sci. 1964 Dec;10:195–225. [PubMed] [Google Scholar]
  11. Ingram F. D., Ingram M. J., Hogben C. A. Quantitative electron probe analysis of soft biologic tissue for electrolytes. J Histochem Cytochem. 1972 Sep;20(9):716–722. doi: 10.1177/20.9.716. [DOI] [PubMed] [Google Scholar]
  12. KAGI J. H., VALEE B. L. Metallothionein: a cadmium- and zinc-containing protein from equine renal cortex. J Biol Chem. 1960 Dec;235:3460–3465. [PubMed] [Google Scholar]
  13. KAGI J. H., VALLEE B. L. Metallothionein: a cadmium and zinc-containign protein from equine renal cortex. II. Physico-chemical properties. J Biol Chem. 1961 Sep;236:2435–2442. [PubMed] [Google Scholar]
  14. Lewis D. C., Ziff M. Intra-articular administration of gold salts. Arthritis Rheum. 1966 Oct;9(5):682–692. doi: 10.1002/art.1780090505. [DOI] [PubMed] [Google Scholar]
  15. Mehard C. W., Volcani B. E. Evaluation of silicon and germanium retention in rat tissues and diatoms during cell and organelle preparation for electron probe microanalysis. J Histochem Cytochem. 1975 May;23(5):348–358. doi: 10.1177/23.5.1127221. [DOI] [PubMed] [Google Scholar]
  16. Norton W. L., Lewis D. C., Ziff M. Electron-dense deposits following injection of gold sodium thiomalate and thiomalic acid. Arthritis Rheum. 1968 Jun;11(3):436–443. doi: 10.1002/art.1780110309. [DOI] [PubMed] [Google Scholar]
  17. Paltemaa S. The inhibition of lysosomal enzymes by gold salts in human synovial fluid cells. Acta Rheumatol Scand. 1968;14(3):161–168. doi: 10.3109/rhe1.1968.14.issue-1-4.18. [DOI] [PubMed] [Google Scholar]
  18. Paltemaa S. The inhibition of lysosomal enzymes by gold salts in human synovial fluid cells. Acta Rheumatol Scand. 1968;14(3):161–168. doi: 10.3109/rhe1.1968.14.issue-1-4.18. [DOI] [PubMed] [Google Scholar]
  19. Persellin R. H., Ziff M. The effect of gold salt on lysosomal enzymes of the peritoneal macrophage. Arthritis Rheum. 1966 Feb;9(1):57–65. doi: 10.1002/art.1780090107. [DOI] [PubMed] [Google Scholar]
  20. Pulido P., Kägi J. H., Vallee B. L. Isolation and some properties of human metallothionein. Biochemistry. 1966 May;5(5):1768–1777. doi: 10.1021/bi00869a046. [DOI] [PubMed] [Google Scholar]
  21. Rodnan G. P., Benedek T. G. The early history of antirheumatic drugs. Arthritis Rheum. 1970 Mar-Apr;13(2):145–165. doi: 10.1002/art.1780130207. [DOI] [PubMed] [Google Scholar]
  22. Stuve J., Galle P. Role of mitochondria in the handling of gold by the kidney. A study by electron microscopy and electron probe microanalysis. J Cell Biol. 1970 Mar;44(3):667–676. doi: 10.1083/jcb.44.3.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. TONNA E. A., BRECHER G., CRONKITE E. P., SCHWARTZ I. L. The autoradiographic localization and distribution of neutron activated gold (Au198) in skeletal tissues and synovia of mice. Arthritis Rheum. 1963 Feb;6:1–10. doi: 10.1002/art.1780060102. [DOI] [PubMed] [Google Scholar]
  24. Wiśniewska J. M., Trojanowska B., Piotrowski J., Jakubowski M. Binding of mercury in the rat kidney by metallothionein. Toxicol Appl Pharmacol. 1970 May;16(3):754–763. doi: 10.1016/0041-008x(70)90081-5. [DOI] [PubMed] [Google Scholar]

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