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. Author manuscript; available in PMC: 2023 Oct 27.
Published in final edited form as: Cell. 2022 Oct 18;185(22):4099–4116.e13. doi: 10.1016/j.cell.2022.09.034

Figure 3: Generation and characterization of Ir25a and Ir76b mutants.

Figure 3:

(A-B) Schematic of the Aedes aegypti Ir76b (A) and Ir25a (B) genomic loci, detailing sgRNA sites and modified protein products of the indicated mutant alleles superimposed on Ir76b and Ir25a protein snake plots, which were generated using Protter v1.0 (Omasits et al., 2014).

(C) Schematic of single stimulus olfactometer assay.

(D-G) Percent of mosquitoes of the indicated genotypes activated to leave the start canister (D-E) or attracted to the indicated stimuli (F-G) in the single stimulus olfactometer assay. Data are displayed as violin plots with median indicated by horizontal black lines and the bounds of the violin corresponding to the range (10–20 mosquitoes/trial, n=6–16 trials). Kruskal-Wallis test was used to compare each mutant allele to wild-type controls (ns, not significant; *p<0.05). See also Supplemental Figure S1.