Fig. 6.

Effects of the WSSP fractions on gluconeogenesis in rat hepatocytes. (A) Glucose production after exposure to WSSP or each of its fractions in isolated hepatocytes. Hepatocytes were incubated for 2 h in the presence of gluconeogenic substrates (pyruvate and lactate) with WSSP or each fraction. Data are expressed as the mean ± standard deviation (n = 6). (B) Expression of PEPCK and G6Pase after exposure to WSSP or each of its fractions in isolated hepatocytes. The lysates of hepatocytes incubated as described above were subjected to Western blot analysis. Representative blot panels of each independent experiment are shown. Intensities of the bands were quantified, and the bar graphs show PEPCK and G6Pase levels corrected by β-actin levels. Data are expressed as the mean ± standard deviation (n = 4). *P < 0.05 vs control. ^†P < 0.001 vs control. ^‡P < 0.001 vs WSSP (10–50 kDa).