Abstract
Crude and purified aqueous extracts of cotton bracts shown to cause airway constriction in naive subjects were assayed for endotoxin content. Pulmonary function measured by flow changes on partial expiratory flow volume curves was used to assess airway responses to the bract extracts after their inhalation by a panel of volunteers. These responses are similar to the acute responses experienced by cotton textile workers. Crude aqueous extracts from various bracts harvested before and after senescence of the cotton plant displayed endotoxin concentrations ranging widely from 0.086 to 50 micrograms/ml. No correlation was found between these differences in endotoxin content and the severity of the airway constrictor response. Purifying the bract extract by a series of procedures that included precipitation of polymeric material by addition of methanol and chromatography on an anion exchange column of DEAE-Sephacel resulted in the elimination of almost all the endotoxin. The partially purified extract contained less than 1 ng/ml of endotoxin. The panel of volunteers responded to this purified bract extract, however, with a decrease in pulmonary function which was more than 60% of that seen with the crude extract of bracts. It is concluded that aqueous extracts of cotton bract contain an agent(s) other than endotoxin that causes acute airway constriction in people.
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Selected References
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- ANTWEILER H. Histamine liberation by cotton dust extracts: evidence against its causation by bacterial endotoxins. Br J Ind Med. 1961 Apr;18:130–132. doi: 10.1136/oem.18.2.130. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bouhuys A. Byssinosis: scheduled asthma in the textile industry. Lung. 1976 Dec 29;154(1):3–16. doi: 10.1007/BF02713514. [DOI] [PubMed] [Google Scholar]
- Bouhuys A., Hunt V. R., Kim B. M., Zapletal A. Maximum expiratory flow rates in induced bronchoconstriction in man. J Clin Invest. 1969 Jun;48(6):1159–1168. doi: 10.1172/JCI106073. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bouhuys A., Mitchell C. A., Schilling R. S., Zuskin E. A physiological study of byssinosis in colonial America. Trans N Y Acad Sci. 1973 Nov;35(7):537–546. doi: 10.1111/j.2164-0947.1973.tb01526.x. [DOI] [PubMed] [Google Scholar]
- Buck M. G., Bouhuys A. Byssinosis: airway constrictor response to cotton bracts. Lung. 1980;158(1):25–32. doi: 10.1007/BF02713699. [DOI] [PubMed] [Google Scholar]
- Carlson R. W., Schaeffer R. C., Jr, Carpio M., Weil M. H. Edema fluid and coagulation changes during fulminant pulmonary edema. Chest. 1981 Jan;79(1):43–49. doi: 10.1378/chest.79.1.43. [DOI] [PubMed] [Google Scholar]
- Cavagna G., Foà V., Vigliani E. C. Effects in man and rabbits of inhalation of cotton dust or extracts and purified endotoxins. Br J Ind Med. 1969 Oct;26(4):314–321. doi: 10.1136/oem.26.4.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cinkotai F. F., Lockwood M. G., Rylander R. Airborne micro-organisms and prevalence of byssinotic symptoms in cotton mills. Am Ind Hyg Assoc J. 1977 Oct;38(10):554–559. doi: 10.1080/0002889778507669. [DOI] [PubMed] [Google Scholar]
- Cinkotai F. F., Lockwood M. G., Rylander R. Airborne micro-organisms and prevalence of byssinotic symptoms in cotton mills. Am Ind Hyg Assoc J. 1977 Oct;38(10):554–559. doi: 10.1080/0002889778507669. [DOI] [PubMed] [Google Scholar]
- Douglas J. S., Duncan P. G., Zuskin E. Characterisation of textile dust extracts: II. Bronchoconstriction in man. Br J Ind Med. 1984 Feb;41(1):70–76. doi: 10.1136/oem.41.1.70. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morey P. R., Sasser P. E., Bethea R. M., Kopetzky M. T. Variation in trash composition in raw cottons. Am Ind Hyg Assoc J. 1976 Jul;37(7):407–412. doi: 10.1080/0002889768507483. [DOI] [PubMed] [Google Scholar]
- PERNIS B., VIGLIANI E. C., CAVAGNA C., FINULLI M. The role of bacterial endotoxins in occupational diseases caused by inhaling vegetable dusts. Br J Ind Med. 1961 Apr;18:120–129. doi: 10.1136/oem.18.2.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rylander R., Imbus H. R., Suh M. W. Bacterial contamination of cotton as an indicator of respiratory effects among card room workers. Br J Ind Med. 1979 Nov;36(4):299–304. doi: 10.1136/oem.36.4.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rylander R., Morey P. Airborne endotoxin in industries processing vegetable fibers. Am Ind Hyg Assoc J. 1982 Nov;43(11):811–812. doi: 10.1080/15298668291410611. [DOI] [PubMed] [Google Scholar]
- Virgulto J., Bouhuys A. Electronic circuits for recording of maximum expiratory flow-volume (MEFV) curves. J Appl Physiol. 1973 Jul;35(1):145–147. doi: 10.1152/jappl.1973.35.1.145. [DOI] [PubMed] [Google Scholar]