Abstract
Castellino, N., and Aloj, S. (1969).Brit. J. industr. Med.,26, 139-143. Intracellular distribution of lead in the liver and kidney of the rat. The distribution of lead in intracellular fractions of liver and kidney has been studied in rats for nine days after intravenous injection of 100 μg. of lead (210Pb) per rat. The radioactivity and the protein nitrogen have been measured in the homogenates and in the nuclei, mitochondria, microsomes, and cell sap. Lead penetrates the cells quickly, being present in all fractions one hour after injection. During the first 72 hours the relative amounts of lead in the different fractions varied, which ruled out the possibility that all the differences could be explained by redistribution after homogenization. The distribution of the metal within the cell probably depends upon differences in the nature and the stability of bonding to intracellular structures. The binding of lead within the mitochondria seemed to be particularly stable, only 24·4% of the radioactivity being removed by washing with 0·25 m sucrose or 0·001 m ethylenediaminetetra-acetate or 0·001 m diethylenetriaminopenta-acetate in 0·25 m sucrose. This finding is discussed in relation to haem biosynthesis within the mitochondrion.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ASHENBRUCKER H., CARTWRIGHT G. E., GOLDBERG A., WINTROBE M. M. Studies on the biosynthesis of heme in vitro by avian erythrocytes. Blood. 1956 Sep;11(9):821–833. [PubMed] [Google Scholar]
- BENARD H., GAJDOS A., GAJDOS-TOROK M. Biogénèse de l'hémoglobine par le sang périphérique du lapin; action du plomb, du fluorure de sodium et de l'oxyde de carbone. Expos Annu Biochim Med. 1958;8(20):41–54. [PubMed] [Google Scholar]
- CASTELLINO N., ALOJ S. KINETICS OF THE DISTRIBUTION AND EXCRETION OF LEAD IN THE RAT. Br J Ind Med. 1964 Oct;21:308–314. doi: 10.1136/oem.21.4.308. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DRESEL E. I., FALK J. E. Studies on the biosynthesis of blood pigments. 3. Haem and porphyrin formation from delta-aminolaevulic acid and from porphobilinogen in haemolysed chicken erythrocytes. Biochem J. 1956 May;63(1):80–87. doi: 10.1042/bj0630080. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ERIKSEN L. Lead intoxication. I. The effect of lead on the in vitro biosynthesis of heme and free erythrocyte porphyrins. Scand J Clin Lab Invest. 1955;7(1):80–85. doi: 10.3109/00365515509134101. [DOI] [PubMed] [Google Scholar]
- GAJDOS A., GAJDOS-TOROK M. Anémie hypochrome, hypersidérémique expérimentale par intoxication saturnine, fluorée ou oxycarbonée du lapin; mecanisme pathogénique. Sang. 1958;29(6):444–460. [PubMed] [Google Scholar]
- GRINSTEIN M., BANNERMAN R. M., MOORE C. V. The utilization of protoporphyrin 9 in heme synthesis. Blood. 1959 Apr;14(4):476–485. [PubMed] [Google Scholar]
- MIANI N., VITERBO B. Studio istoautoradiografico sulla localizzazione del piombo (RaD) in vari organl di cane. Z Zellforsch Mikrosk Anat. 1958;49(2):188–208. [PubMed] [Google Scholar]
- MINAKAMI S., YONEYAMA Y., YOSHIKAWA H. On the biosynthesis of heme and hemeproteins in liver cell. Biochim Biophys Acta. 1958 May;28(2):447–449. doi: 10.1016/0006-3002(58)90500-6. [DOI] [PubMed] [Google Scholar]
- PASSOW H., ROTHSTEIN A., CLARKSON T. W. The general pharmacology of the heavy metals. Pharmacol Rev. 1961 Jun;13:185–224. [PubMed] [Google Scholar]
- TISHKOFF G. H., GRANVILLE N. B., ROSEN R., DAMESHEK W. Excretion of delta-aminolevulinic acid in lead intoxication. Acta Haematol. 1958 Jun;19(6):321–326. doi: 10.1159/000205451. [DOI] [PubMed] [Google Scholar]