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. 2023 Apr 4;28(7):3214. doi: 10.3390/molecules28073214

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Annexin V-FITC analysis of PC-3 cells treated with GMG-ITC (3.5 µg/mL) for 24 (B), 48 (C), and 72 (D) hours, with (A) representing the untreated cells. Early apoptosis (Annexin+/PI−) is shown in the lower right quadrant (Q4) for each panel while late apoptosis (Annexin+/PI+) is shown in the upper right quadrant (Q2). Viable cells are represented in the lower left quadrant (Q3). Necrosis (Annexin−/PI+) is shown in the upper left quadrant (Q1). Results represent one of three independent experiments.