Skip to main content
PMC home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1995 Jul;54(7):597–600. doi: 10.1136/ard.54.7.597

Persistence of parvovirus B19 in synovial fluid and bone marrow.

S Nikkari 1, A Roivainen 1, P Hannonen 1, T Möttönen 1, R Luukkainen 1, T Yli-Jama 1, P Toivanen 1
PMCID: PMC1009942  PMID: 7668905

Abstract

OBJECTIVES--To determine whether parvovirus B19 (B19) persists in rheumatoid arthritis (RA). METHODS--Polymerase chain reaction (PCR) was used to detect parvovirus B19 genome in the synovial fluid cells or peripheral blood mononuclear cells from 61 patients with early RA; bone marrow from one patient was also studied. The synovium or synovial fluid cells from 28 patients with advanced RA, and synovial fluid cell samples from 18 patients with reactive arthritis (as controls) were studied. Two separate sets of primers and probe were used. RESULTS--Parvovirus B19 specific gene sequences were detected in two patients with early arthritis fulfilling the criteria for RA. CONCLUSION--Parvovirus B19 does not play a significant role in the aetiopathogenesis of RA. However, a few cases of a disease indistinguishable from RA may be triggered by parvovirus B19 infection.

Full text

PDF
597

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson M. J., Jones S. E., Minson A. C. Diagnosis of human parvovirus infection by dot-blot hybridization using cloned viral DNA. J Med Virol. 1985 Feb;15(2):163–172. doi: 10.1002/jmv.1890150209. [DOI] [PubMed] [Google Scholar]
  2. Dijkmans B. A., van Elsacker-Niele A. M., Salimans M. M., van Albada-Kuipers G. A., de Vries E., Weiland H. T. Human parvovirus B19 DNA in synovial fluid. Arthritis Rheum. 1988 Feb;31(2):279–281. doi: 10.1002/art.1780310218. [DOI] [PubMed] [Google Scholar]
  3. Finkel T. H., Török T. J., Ferguson P. J., Durigon E. L., Zaki S. R., Leung D. Y., Harbeck R. J., Gelfand E. W., Saulsbury F. T., Hollister J. R. Chronic parvovirus B19 infection and systemic necrotising vasculitis: opportunistic infection or aetiological agent? Lancet. 1994 May 21;343(8908):1255–1258. doi: 10.1016/s0140-6736(94)92152-0. [DOI] [PubMed] [Google Scholar]
  4. Foto F., Saag K. G., Scharosch L. L., Howard E. J., Naides S. J. Parvovirus B19-specific DNA in bone marrow from B19 arthropathy patients: evidence for B19 virus persistence. J Infect Dis. 1993 Mar;167(3):744–748. doi: 10.1093/infdis/167.3.744. [DOI] [PubMed] [Google Scholar]
  5. Frickhofen N., Young N. S. Persistent parvovirus B19 infections in humans. Microb Pathog. 1989 Nov;7(5):319–327. doi: 10.1016/0882-4010(89)90035-1. [DOI] [PubMed] [Google Scholar]
  6. Jackson D. P., Lewis F. A., Taylor G. R., Boylston A. W., Quirke P. Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction. J Clin Pathol. 1990 Jun;43(6):499–504. doi: 10.1136/jcp.43.6.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kandolf R., Kirschner P., Hofschneider P. H., Vischer T. L. Detection of parvovirus in a patient with "reactive arthritis" by in situ hybridization. Clin Rheumatol. 1989 Sep;8(3):398–401. doi: 10.1007/BF02030355. [DOI] [PubMed] [Google Scholar]
  8. Naides S. J. Parvovirus B19 infection. Rheum Dis Clin North Am. 1993 May;19(2):457–475. [PubMed] [Google Scholar]
  9. Nikkari S., Luukkainen R., Möttönen T., Meurman O., Hannonen P., Skurnik M., Toivanen P. Does parvovirus B19 have a role in rheumatoid arthritis? Ann Rheum Dis. 1994 Feb;53(2):106–111. doi: 10.1136/ard.53.2.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nikkari S., Merilahti-Palo R., Saario R., Söderström K. O., Granfors K., Skurnik M., Toivanen P. Yersinia-triggered reactive arthritis. Use of polymerase chain reaction and immunocytochemical staining in the detection of bacterial components from synovial specimens. Arthritis Rheum. 1992 Jun;35(6):682–687. doi: 10.1002/art.1780350613. [DOI] [PubMed] [Google Scholar]
  11. Nikkari S., Mertsola J., Korvenranta H., Vainionpä R., Toivanen P. Wegener's granulomatosis and parvovirus B19 infection. Arthritis Rheum. 1994 Nov;37(11):1707–1708. doi: 10.1002/art.1780371122. [DOI] [PubMed] [Google Scholar]
  12. Saal J. G., Steidle M., Einsele H., Müller C. A., Fritz P., Zacher J. Persistence of B19 parvovirus in synovial membranes of patients with rheumatoid arthritis. Rheumatol Int. 1992;12(4):147–151. doi: 10.1007/BF00274934. [DOI] [PubMed] [Google Scholar]
  13. Tyndall A., Jelk W., Hirsch H. H. Parvovirus B19 and erosive polyarthritis. Lancet. 1994 Feb 19;343(8895):480–481. doi: 10.1016/s0140-6736(94)92725-1. [DOI] [PubMed] [Google Scholar]
  14. White D. G., Woolf A. D., Mortimer P. P., Cohen B. J., Blake D. R., Bacon P. A. Human parvovirus arthropathy. Lancet. 1985 Feb 23;1(8426):419–421. doi: 10.1016/s0140-6736(85)91145-6. [DOI] [PubMed] [Google Scholar]
  15. Zhang L., Nikkari S., Skurnik M., Ziegler T., Luukkainen R., Möttönen T., Toivanen P. Detection of herpesviruses by polymerase chain reaction in lymphocytes from patients with rheumatoid arthritis. Arthritis Rheum. 1993 Aug;36(8):1080–1086. doi: 10.1002/art.1780360808. [DOI] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES