Plant Biology Correction for “Molecular basis of locus-specific H3K9 methylation catalyzed by SUVH6 in plants,” by Jian Zhang, Jianlong Yuan, Juncheng Lin, Lixian Chen, Li-Yuan You, Shuling Chen, Li Peng, Chun-Han Wang, Jiamu Du, and Cheng-Guo Duan, which published December 29, 2022; 10.1073/pnas.2211155120 (Proc. Natl. Acad. Sci. U.S.A. 120, e2211155120).
The authors note that Fig. 7 appeared incorrectly. The sample names “Wild-type” and “ossuvh6” in Fig. 7E were reversed. The corrected figure and its legend appear below. The online version has been corrected.
Fig. 7.
SUVH6-ASI1 module-dependent locus-specific H3K9me2 deposition is conserved in plants. (A and B) ChIP-qPCR showing the fold enrichment of OsSUVH6 (A) and H3K9me2 (B) at the intronic TRE regions of two representative APA genes. OsSUVH6 ChIP assays were performed in wild-type and OsSUVH6-Flag transgenic rice plants. H3K9me2 ChIP assays were performed in wild-type, osasi1 and ossuvh6 plants. ChIP–qPCR data were normalized to internal inputs, and then a control gene (Osactin) was used to calculate relative ChIP signals. The data are the means ± SD of three biological repeats. An unpaired one-tailed t test was performed. ***, **, and * represent P values <0.01, 0.1, and 0.5, respectively. (C) The effect of ossuvh6 and osasi1 mutations on the global polyadenylation patterns in rice. Top2 expressed poly(A) sites were extracted for the analyses. Venn diagram shows that the genes were corporately regulated by OsASI1 and OsSUVH6 (Upper). Black rectangles indicate sites with expression variation <20% when comparing to the site in wild-type (Lower). (D) Snapshots of PAT-seq showing the usage of poly(A) sites at two intronic TRE-containing genes. (E) Heatmap showing the expression pattern of differentially expressed genes in ossuvh6 mutant and wild-type plants. (F) Venn diagram showing the overlap between the species encoding the homologous proteins of ASI1, SUVH6, and SUVH family histone MTases. Species analyses were conducted using an online tool (http://www.pantherdb.org/). (G) A working model of SUVH6-ASI1 module-dependent H3K9me2 deposition and dependent gene expression regulation. The cylinder diagrams represent exons.