Neuroscience Correction for “Cerebellar plasticity and associative memories are controlled by perineuronal nets,” by Daniela Carulli, Robin Broersen, Fred de Winter, Elizabeth M. Muir, Maja Mešković, Matthijs de Waal, Sharon de Vries, Henk-Jan Boele, Cathrin B. Canto, Chris I. De Zeeuw, and Joost Verhaagen, which was first published March 9, 2020; 10.1073/pnas.1916163117 (Proc. Natl. Acad. Sci. U.S.A. 117, 6855–6865).
The authors note that due to an error in figure preparation, Fig. 5M was duplicated into Fig. 5O. The corrected figure and its legend appear below. The online version has been corrected.
Fig. 5.
Effect of ch’ase on plasticity of GABAergic terminals in the DCN. (A–H) Immunolabeling for VGAT and calbindin (CALB) in the IntA of LV-PGK-GFP (A–D) and LV-PGK-ch’ase (E–H) injected mice at 4 wk after virus injection. GABAergic terminals, including Purkinje cell boutons, appear as discrete puncta (abundant around DCN neuronal somata) in GFP-injected mice (A–D). See also SI Appendix, Fig. S3. After ch’ase, they show a more continuous distribution (E–H). See also SI Appendix, Fig. S4. (I) Representative electron micrograph of a control DCN neuron (no LV-ch’ase injection), surrounded by GABAergic terminals (digitally blue color coded). See also SI Appendix, Fig. S5. (J) GABAergic terminals (blue) in uninjected (uninj.) DCN. (K) GABAergic terminals (blue) in ch’ase-injected DCN. (L–Q) Quantitations in EM pictures. (L) Density (number per mm neuronal membrane) of GABAergic terminals. Segments of neuronal membrane are shown as individual elements. (M) Size of GABAergic terminals (individual terminals are shown). (N) Frequency distribution of terminals according to size. (O) Number of release sites per μm of membrane of synaptic terminal adjacent to the postsynaptic neuron (individual terminals are shown). (P) Length of individual release sites. (Q) Fraction of the area occupied by mitochondria in individual GABA+synaptic terminals. (R–S′′) Immunolabeling with anti-gephyrin antibodies (red) shows gephyrin+clusters juxtaposed to GABAergic terminals (which were stained by anti-VGAT antibodies; green) around DCN neurons in LV-GFP (R–R′′) and LV-ch’ase mice (S–S′′). WFA (blue) reveals the PNN around a control neuron (R) but is not visible around a ch’ase-treated neuron(S). (T) Number of gephyrin+clusters per μm neuronal membrane (individual membrane segments are shown). *P < 0.05, ***P < 0.001. [Scale bar, 20 μmin A (also applies to B, C, and E–G), 5 μmin D (also applies to H) and I, 0.5 μmin J (also applies to K], 5 μmin R (also applies to R′, S, and S′), and 2.5 μmin R′′ (also applies to S′′).]