In the published article, there was an error in the legend for Figure 3 as published. This sentence previously stated:
“The consequences of BTL-1 on the expression of cytokines were determined by E 50 μM) for 24 h and then treated with LPS (50 μm/mL) for another 1 h.”
The corrected legend appears below.
“Impact of BTL-1 on inflammatory cytokines production in LPS-induced IPEC-J2 cell. The outcome of BTL-1 on IPEC-J2 cell viability (A,B). The consequences of BTL-1 on the expression of cytokines were determined by ELISA (C–F) and qPCR (G–J). The consequences of BTL-1 on the expression of cytokines were determined by ELISA. The cells were treated with BTL-1 (10, 20, and 50 μM) for 24 h and then treated with LPS (50 μg/mL) for another 1 h. The data shown are representative of three independent experiments. The results were expressed as the means ± SEM. #P < 0.05, ##P < 0.01, compared to non-treated group, *P < 0.05, **P < 0.01 compared to LPS group.”
In the published article, there was an error in Table 1 as published. Since our qPCR primers are designed by Shanghai Sangong Biotechnology Co., Ltd., there were a large number of primer sequences in the same account, which led to our submission error. At the same time, we also found that the primer of β-actin gene was missing. The corrected Table 1 and its caption appear below.
Table 1.
Primer sequence.
| Gene ID | Primer | Sequence (5'-3') |
|---|---|---|
| 414396 | pig β-actin Forward | GCTGTCCCTGTATGCCTCT |
| pig β-actin Reverse | GATGTCACGCACGATTTCC | |
| 399500 | pig IL-6 Forward | ATAAGGGAAATGTCGAGGCTGTGC |
| pig IL-6 Reverse | GGGTGGTGGCTTTGTCTGGATTC | |
| 397122 | pig IL-1β Forward | CAAGCCAGAGAAGCAAGGTGTCC |
| pig IL-1β Reverse | GCCGTCCTCAGCAGCAAGAAG | |
| 397106 | pig IL-10 Forward | AGCCAGCATTAAGTCTGAGAACAGC |
| pig IL-10 Reverse | GGTCAGCAACAAGTCGCCCATC | |
| 397086 | pig TNF-α Forward | AAAGGACACCATGAGCACGGAAAG |
| pig TNF-α Reverse | CGCCACGAGCAGGAATGAGAAG | |
| 399541 | pig TLR4 Forward | GCCATCGCTGCTAACATCATC |
| pig TLR4 Reverse | CTCATACTCAAAGATACACCATCGG | |
| 100135665 | pig P65 Forward | CTGAGGCTATAACTCGCTTGGTGAC |
| pig P65 Reverse | CATGTCCGCAATGGAGGAGAAGTC | |
| 100157586 | pig IκB Forward | GGCAGTGACATGAGTGGCAGATAC |
| pig IκB Reverse | CTTCCTTGAGGCTGTGCTTCTTCC | |
| 445013 | pig ERK1 Forward | ACGTCATTGGCATCCGAGACATTC |
| pig ERK1 Reverse | GAGGAAGTAGCAGATGTGGTCGTTG | |
| 100626611 | pig P38 Forward | CGCCTGTGAAGACCTCCTTGAAC |
| pig P38 Reverse | TTCCTGTCCTCCACCTTCCGAAG | |
| 396610 | pig JNK Forward | ACTACAGAGCACCTGAGGTCATCC |
| pig JNK Reverse | ATTTCTCCCATAATGCACCCCACAG | |
| 397236 | pig Occludin Forward | CAGTGGTAACTTGGAGGCGTCTTC |
| pig Occludin Reverse | CGTCGTGTAGTCTGTCTCGTAATGG | |
| 100736682 | pig ZO-1 Forward | CCAGGGAGAGAAGTGCCAGTAGG |
| pig ZO-1 Reverse | TTTGGTGGGTTTGGTGGGTTGAC | |
| 100625166 | pig Claudin1 Forward | AGAAGATGCGGATGGCTGTCATTG |
| pig Claudin1 Reverse | ACCATACCATGCTGTGGCAACTAAG | |
| 11461 | mouse β-actin Forward | GTGACGTTGACATCCGTAAAGA |
| mouse β-actin Reverse | GCCGGACTCATCGTACTCC | |
| 16193 | mouse IL-6 Forward | CTCCCAACAGACCTGTCTATAC |
| mouse IL-6 Reverse | CCATTGCACAACTCTTTTCTCA | |
| 16176 | mouse IL-1β Forward | GAAATGCCACCTTTTGACAGTG |
| mouse IL-1β Reverse | TGGATGCTCATCAGGACAG | |
| 16153 | mouse IL-10 Forward | TTCTTTCAAACAAAGGACCAGC |
| mouse IL-10 Reverse | GCAACCCAAGTAACCCTTAAAG | |
| 21926 | mouse TNF-α Forward | ATGTCTCAGCCTCTTCTCATTC |
| mouse TNF-α Reverse | GCTTGTCACTCGAATTTTGAGA | |
| 21898 | mouse TLR4 Forward | GCCATCATTATGAGTGCCAATT |
| mouse TLR4 Reverse | AGGGATAAGAACGCTGAGAATT | |
| 19697 | mouse P65 Forward | TGCGATTCCGCTATAAATGCG |
| mouse P65 Reverse | ACAAGTTCATGTGGATGAGGC | |
| 12675 | mouse IκB Forward | TTGGGTTATGCCAAAGATGTTG |
| mouse IκB Reverse | GCTGTGTACGGCTTATTTTCAA | |
| 26417 | mouse ERK1 Forward | CAGCTCAACCACATTCTAGGTA |
| mouse ERK1 Reverse | TCAAGAGCTTTGGAGTCAGATT | |
| 26416 | mouse P38 Forward | AGGAATTCAATGACGTGTACCT |
| mouse P38 Reverse | AGGTCCCTGTGAATTATGTCAG | |
| 26419 | mouse JNK1 Forward | TTGAAAACAGGCCTAAATACGC |
| mouse JNK1 Reverse | GTTTGTTATGCTCTGAGTCAGC | |
| 18260 | mouse Occludin Forward | TGCTTCATCGCTTCCTTAGTAA |
| mouse Occludin Reverse | GGGTTCACTCCCATTATGTACA | |
| 21872 | mouse ZO-1 Forward | CTGGTGAAGTCTCGGAAAAATG |
| mouse ZO-1 Reverse | CATCTCTTGCTGCCAAACTATC | |
| 12737 | mouse Claudin1 Forward | AGATACAGTGCAAAGTCTTCGA |
| mouse Claudin1 Reverse | CAGGATGCCAATTACCATCAAG |
In the published article, there was an error in Figure 10 as published. Due to the poor shooting effect of some tissue sections, we adjusted the angle of the sections, and the remarks were not clear enough, resulting in mistakenly uploading two images of the same tissue section, namely, the HE staining sections of the low dose group and the medium dose group. The corrected Figure 10 and its caption appear below.
Figure 10.
The effect of BTL-1 on the intestinal injury of IBD mouse model induced by DSS. Histopathological changes after DSS stimulation in the colon (A). The in vitro intestinal barrier function evaluated by FITC-D fluorescence intensity (B). Upregulation of BTL-1 on tight junction proteins as determined by qPCR (C–E) and western blotting (F–I). The results were expressed as the means ± SEM of n = 3. #P < 0.05, ##P < 0.01, compared to control group, *P < 0.05, **P < 0.01 compared to DSS group.
In the published article, there was an error in Materials and Methods, Subsection “Induction of Experimental Colitis.” This sentence previously stated:
“The animals were randomly allocated to one of the following five groups (n = 5): normal control (only given food and water without DSS), DSS model (administered DSS in drinking water), low-concentration BTL-1 (administered 10 mg/kg DSS, p.o), middle-concentration BTL-1 (taken 20 mg/kg DSS, p.o), and high-concentration BTL-1 group (given 50 mg/kg DSS, p.o) group.”
The corrected sentence appears below:
“The animals were randomly allocated to one of the following five groups (n = 5): normal control (only given food and water without DSS), DSS model (administered DSS in drinking water), low-concentration BTL-1 (administered 1 mg/kg BTL-1, p.o), middle-concentration BTL-1 (taken 2 mg/kg BTL-1, p.o), and high-concentration BTL-1 group (given 5 mg/kg BTL-1, p.o) group.”
The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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