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. 2023 Apr 14;41(20):3233–3246. doi: 10.1016/j.vaccine.2023.04.020

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© 2023 The Author(s)

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 2 — Distribution of Ad5 vector DNA to the spleen and liver. Spleen (A) and liver (B) samples were collected from C57BL/6 mice (n = 4) at 1, 3, 7 or 30 days following either intranasal (i.n.) or intramuscular (i.m.) administration of Ad-RBD (10⁹ virus particles). Ad5-RBD vector was quantified from genomic DNA samples (25 ng) by real-time PCR using a specific probe-based assay to detect the RBD. Vector copy numbers were determined by comparison to plasmid standard (pAdapt-RBD). Individual values and means ± SEM are plotted.