Fig. 6.

Upregulation of selected immune genes, differentially expressed in RBD-stimulated spleen cells from mice treated with Ad5-RBD administered intranasally (i.n.) or intramuscularly (i.m.). Groups of female HLA-DQ6 mice (n = 3) were inoculated once with Ad5-RBD at doses ranging from 10⁵-10¹⁰ viral particles/mouse. After 25 days, spleen cells were restimulated in vitro with recombinant SARS-CoV-2 spike protein receptor binding domain (RBD). Cells were harvested after 3 days of culture, and bulk RNA was isolated for transcriptomic analyses (RNA sequencing; compare Fig. 5). Panels depict the results for 9 selected immune genes [T helper 1/cytotoxic T cell markers: Interferon gamma (Ifng), perforin 1 (Prf1), granzyme B (Gzmb); Interferon gamma-related genes: Interferon regulatory factor 1 (Irf1), signal transducer and activator of transcription 1 (Stat1), chemokine (C-X-C motif) ligand 9 (CXCL9); Lymphocyte activation-related genes: Interleukin 2-receptor alpha (Il2ra), chemokine (C motif) ligand (XCL1), thymocyte differentiation antigen 1 (Thy1)]. Results expressed as read counts.