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. Author manuscript; available in PMC: 2023 Oct 5.
Published in final edited form as: Nature. 2022 Oct 5;610(7931):389–393. doi: 10.1038/s41586-022-05278-9

Extended Data Fig. 5 |. Kemp elimination catalyzed by AlleyCat10 in presence of Ca2+ (black) and in absence of Ca2+ (red).

Extended Data Fig. 5 |

The activity of 0.1 μM AlleyCat10 was tested with 0.12–0.96 mM substrate in 20 mM HEPES, 100 mM NaCl (pH 7.0) at 22°C with 10 mM CaCl2 (black) or 100 μM EDTA (red).