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. 1985 Feb;61(1):1–6. doi: 10.1136/sti.61.1.1

Monoclonal antibodies to Treponema pallidum: recognition of a major polypeptide antigen.

R W Thornburg, J Morrison-Plummer, J B Baseman
PMCID: PMC1011746  PMID: 3910538

Abstract

Hybridomas secreting monoclonal antibodies that reacted with a 45 000 dalton surface polypeptide and major immunogen of T pallidum were produced. This polypeptide was also found in T pertenue but not in T hyodysenteriae or T phagedenis biotype Reiter.

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Selected References

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  1. Alderete J. F., Baseman J. B. Analysis of serum IgG against Treponema pallidum protein antigens in experimentally infected rabbits. Br J Vener Dis. 1981 Oct;57(5):302–308. doi: 10.1136/sti.57.5.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alderete J. F., Baseman J. B. Surface characterization of virulent Treponema pallidum. Infect Immun. 1980 Dec;30(3):814–823. doi: 10.1128/iai.30.3.814-823.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alderete J. F., Baseman J. B. Surface-associated host proteins on virulent Treponema pallidum. Infect Immun. 1979 Dec;26(3):1048–1056. doi: 10.1128/iai.26.3.1048-1056.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baseman J. B., Hayes E. C. Molecular characterization of receptor binding proteins and immunogens of virulent Treponema pallidum. J Exp Med. 1980 Mar 1;151(3):573–586. doi: 10.1084/jem.151.3.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Baughn R. E., Adams C. B., Musher D. M. Circulating immune complexes in experimental syphilis: identification of treponemal antigens and specific antibodies to treponemal antigens in isolated complexes. Infect Immun. 1983 Nov;42(2):585–593. doi: 10.1128/iai.42.2.585-593.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bishop N. H., Miller J. N. Humoral immunity in experimental syphilis. I. The demonstration of resistance conferred by passive immunization. J Immunol. 1976 Jul;117(1):191–196. [PubMed] [Google Scholar]
  7. Bishop N. H., Miller J. N. Humoral immunity in experimental syphilis. II. The relationship of neutralizing factors in immune serum to acquired resistance. J Immunol. 1976 Jul;117(1):197–207. [PubMed] [Google Scholar]
  8. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  9. Fitzgerald T. J. Pathogenesis and immunology of Treponema pallidum. Annu Rev Microbiol. 1981;35:29–54. doi: 10.1146/annurev.mi.35.100181.000333. [DOI] [PubMed] [Google Scholar]
  10. Hayes N. S., Muse K. E., Collier A. M., Baseman J. B. Parasitism by virulent Treponema pallidum of host cell surfaces. Infect Immun. 1977 Jul;17(1):174–186. doi: 10.1128/iai.17.1.174-186.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kinyon J. M., Harris D. L. Growth in Treponema hyodysenteriae in liquid medium. Vet Rec. 1974 Sep 7;95(10):219–220. doi: 10.1136/vr.95.10.219. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Lukehart S. A., Baker-Zander S. A., Gubish E. R., Jr Identification of Treponema pallidum antigens: comparison with a nonpathogenic treponeme. J Immunol. 1982 Aug;129(2):833–838. [PubMed] [Google Scholar]
  15. March S. C., Parikh I., Cuatrecasas P. A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal Biochem. 1974 Jul;60(1):149–152. doi: 10.1016/0003-2697(74)90139-0. [DOI] [PubMed] [Google Scholar]
  16. Marchalonis J. J., Cone R. E., Santer V. Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes. Biochem J. 1971 Oct;124(5):921–927. doi: 10.1042/bj1240921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Miao R. M., Fieldsteel A. H. Genetic relationship between Treponema pallidum and Treponema pertenue, two noncultivable human pathogens. J Bacteriol. 1980 Jan;141(1):427–429. doi: 10.1128/jb.141.1.427-429.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morrison-Plummer J., Alderete J. F., Baseman J. B. Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum. Br J Vener Dis. 1983 Apr;59(2):75–79. doi: 10.1136/sti.59.2.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Morrison-Plummer J., Jones D. H., Baseman J. B. An ELISA to detect monoclonal antibodies specific for lipid determinants of Mycoplasma pneumoniae. J Immunol Methods. 1983 Nov 11;64(1-2):165–178. doi: 10.1016/0022-1759(83)90395-2. [DOI] [PubMed] [Google Scholar]
  21. Pedersen N. S., Axelsen N. H., Petersen C. S. Antigenic analysis of Treponema pallidum: cross-reactions between individual antigens of T. pallidum and T. Reiter. Scand J Immunol. 1981;13(2):143–150. doi: 10.1111/j.1365-3083.1981.tb00120.x. [DOI] [PubMed] [Google Scholar]
  22. Peterson K. M., Baseman J. B., Alderete J. F. Treponema pallidum receptor binding proteins interact with fibronectin. J Exp Med. 1983 Jun 1;157(6):1958–1970. doi: 10.1084/jem.157.6.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Robertson S. M., Kettman J. R., Miller J. N., Norgard M. V. Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols). Infect Immun. 1982 Jun;36(3):1076–1085. doi: 10.1128/iai.36.3.1076-1085.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Saunders J. M., Folds J. D. Development of monoclonal antibodies that recognize Treponema pallidum. Infect Immun. 1983 Aug;41(2):844–847. doi: 10.1128/iai.41.2.844-847.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sell S., Gamboa D., Baker-Zander S. A., Lukehart S. A., Miller J. N. Host response to Treponema pallidum in intradermally-infected rabbits: evidence for persistence of infection at local and distant sites. J Invest Dermatol. 1980 Dec;75(6):470–475. doi: 10.1111/1523-1747.ep12524230. [DOI] [PubMed] [Google Scholar]
  26. Swanstrom R., Shank P. R. X-Ray Intensifying Screens Greatly Enhance the Detection by Autoradiography of the Radioactive Isotopes 32P and 125I. Anal Biochem. 1978 May;86(1):184–192. doi: 10.1016/0003-2697(78)90333-0. [DOI] [PubMed] [Google Scholar]
  27. Thornburg R. W., Baseman J. B. Comparison of major protein antigens and protein profiles of Treponema pallidum and Treponema pertenue. Infect Immun. 1983 Nov;42(2):623–627. doi: 10.1128/iai.42.2.623-627.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Weiser R. S., Erickson D., Perine P. L., Pearsall N. N. Immunity to syphilis: passive transfer in rabbits using serial doses of immune serum. Infect Immun. 1976 May;13(5):1402–1407. doi: 10.1128/iai.13.5.1402-1407.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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