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. 1985 Apr;61(2):75–81. doi: 10.1136/sti.61.2.75

Experimental infection of inbred guinea pigs with Treponema pallidum: development of lesions and formation of antibodies.

C S Pavia, C J Niederbuhl
PMCID: PMC1011771  PMID: 3884485

Abstract

Inbred strain 2 and strain 13 guinea pigs were infected intradermally in the hind legs with different numbers of the virulent Nichols strain of Treponema pallidum. About 91% of the animals developed clearly visible lesions after being injected with 5 X 10(6) to 10 X 10(6) treponemes. T pallidum organisms could be isolated from skin lesions at various stages after infection. Infected animals were monitored for the production of specific treponemal and non-specific cardiolipin antibodies by the fluorescent treponemal antibody (FTA) and microhaemagglutination (MHA-TP) tests and the rapid plasma reagin (RPR) card test. Low levels of specific antibodies could be detected by both FTA and MHA-TP tests from three to four weeks after infection. Maximum titres of treponemal antibody generally occurred after week 6 and persisted for several more months. These peak titres ranged from 1/40 to 1/80 in the FTA test and 1/160 to 1/320 in the MHA-TP test. During the same period infected guinea pigs, unlike rabbits with syphilis, did not produce detectable quantities of antibodies against cardiolipin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fieldsteel A. H., Becker F. A., Stout J. G. Prolonged survival of virulent Treponema pallidum (Nichols strain) in cell-free and tissue culture systems. Infect Immun. 1977 Oct;18(1):173–182. doi: 10.1128/iai.18.1.173-182.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Folds J. D., Rauchbach A. S., Shores E., Saunders J. M. Evaluation of the inbred mouse as a model for experimental Treponema pallidum infection. Scand J Immunol. 1983 Sep;18(3):201–206. doi: 10.1111/j.1365-3083.1983.tb00858.x. [DOI] [PubMed] [Google Scholar]
  3. GASTINEL P., VAISMAN A., VAISMAN La dispersion tréponémique dans la syphilis expérimentale du cobaye. Ann Dermatol Syphiligr (Paris) 1955 Sep-Oct;82(5):481–483. [PubMed] [Google Scholar]
  4. HUNTER E. F., DEACON W. E., MEYER P. E. AN IMPROVED FTA TEST FOR SYPHILIS, THE ABSORPTION PROCEDURE (FTA-ABS). Public Health Rep. 1964 May;79:410–412. [PMC free article] [PubMed] [Google Scholar]
  5. Klein J. R., Monjan A. A., Hardy P. H., Jr, Cole G. A. Abrogation of genetically controlled resistance of mice to Treponema pallidum by irradiation. Nature. 1980 Feb 7;283(5747):572–574. doi: 10.1038/283572a0. [DOI] [PubMed] [Google Scholar]
  6. March R. W., Stiles G. E. The reagin screen test: a new reagin card test for syphilis. Sex Transm Dis. 1980 Apr-Jun;7(2):66–70. doi: 10.1097/00007435-198004000-00006. [DOI] [PubMed] [Google Scholar]
  7. Norris S. J. In vitro cultivation of Treponema pallidum: independent confirmation. Infect Immun. 1982 Apr;36(1):437–439. doi: 10.1128/iai.36.1.437-439.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pavia C. S., Diggs C. L., Williams J. The use of metrizamide for isopyknic separation and enrichment of Plasmodium falciparum schizonts from continuous culture. Am J Trop Med Hyg. 1983 Jul;32(4):675–681. doi: 10.4269/ajtmh.1983.32.675. [DOI] [PubMed] [Google Scholar]
  9. Pavia C. S., Folds J. D., Baseman J. B. Selective in vitro response of thymus-derived lymphocytes from Treponema pallidum-infected rabbits. Infect Immun. 1977 Dec;18(3):603–611. doi: 10.1128/iai.18.3.603-611.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pepose J. S., Bishop N. H., Feigenbaum S., Miller J. N., Zeltzer P. M. The humoral immune response in rabbits infected with Treponema pallidum: Comparison of antibody levels measured by the staphylococcal protein A-IgG (SPA-TP) microassay with VDRL, FTA-Abs, and TPI antibody responses during the development of acquired resistance to challenge. Sex Transm Dis. 1980 Jul-Sep;7(3):125–129. [PubMed] [Google Scholar]
  11. Pierce C. S., Wicher K., Nakeeb S. Experimental syphilis: guinea pig model. Br J Vener Dis. 1983 Jun;59(3):157–168. doi: 10.1136/sti.59.3.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schell R. F., Chan J. K., Le Frock J. L. Endemic syphilis: passive transfer of resistance with serum and cells in hamsters. J Infect Dis. 1979 Sep;140(3):378–383. doi: 10.1093/infdis/140.3.378. [DOI] [PubMed] [Google Scholar]
  13. Schell R. F., LeFrock J. L., Chan J. K., Bagasra O. LSH hamster model of syphilitic infection. Infect Immun. 1980 Jun;28(3):909–913. doi: 10.1128/iai.28.3.909-913.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schell R. F., Musher D. M., Jacobson K., Schwethelm P. New evidence for the non-infectivity of Treponema pallidum for mice. Br J Vener Dis. 1975 Feb;51(1):19–21. doi: 10.1136/sti.51.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wicher K., Wicher V., Wang M. C. Cellular and humoral immune response to guinea pig infected with Treponema pallidum. Int Arch Allergy Appl Immunol. 1976;51(3):284–297. doi: 10.1159/000231603. [DOI] [PubMed] [Google Scholar]

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