Table 1.
Differential abundance of cell wall-modifying enzymes in cells and MVs of P. aeruginosa PAO1 upon MMC treatment. Predicted localization of proteins: C, cytoplasm; IM, inner membrane associated; P, periplasm; OM, outer membrane associated; E, extracellular.
| Gene number | Gene name | Protein description | Size (kDa) | Possible localization | Fold increase | |
|---|---|---|---|---|---|---|
| Cells | MVs | |||||
| PA0378 | mtgA | Biosynthetic peptidoglycan transglycosylase | 26.5 | IM | < 2.0 | 11.2 |
| PA0629 | lys | Endolysin | 23.0 | P | 27.4 | 2.1 |
| PA0807 | ampDh3 | AmpDh3 | 28.7 | P | 98.0 | 2.9 |
| PA0869 | pbpG (PBP7) | D-alanyl-D-alanine endopeptidase | 34.0 | P | 30.5 | < 2.0 |
| PA1171 | sltB2 (sltG) | Soluble lytic transglycolase | 42.7 | IM | 6.8 | 3.7 |
| PA1222 | mltA | Membrane-bound lytic murein transglycosylase A | 41.9 | OM | 5.9 | 20.4 |
| PA2854 | erfK | Putative L,D-transpeptidase | 34.8 | P | 2.5 | < 2.0 |
| PA2963 | mltG | Endolytic murein transglycosylase | 39.6 | IM | < 2.0 | 5.5 |
| PA3020 | mltC | Probable soluble lytic transglycosylase | 73.4 | P | 5.7 | < 2.0 |
| PA3047 | dacB (PBP4) | Probable D-alanyl-D-alanine carboxypeptidase | 51.9 | P | < 2.0 | 14.1 |
| PA3484 | tse3 | Muramidase (toxin of Type VI secretion system) | 44.4 | E | 11.6 | < 2.0 |
| PA3764 | mltF | Membrane-bound lytic murein transglycosylase F | 55.2 | OM | 3.0 | 26.6 |
| PA3992 | sltB3 (sltH) | Soluble lytic (exolytic) transglycosylase | 47.8 | IM | 3.1 | 3.7 |
| PA4000 | rlpA | Endolytic peptidoglycan transglycosylase RlpA | 36.5 | IM | < 2.0 | 7.0 |
| PA4001 | sltB1 | Soluble lytic transglycosylase B | 37.9 | IM | < 2.0 | 16.1 |
| PA4003 | pbpA | Peptidoglycan D,D-transpeptidase MrdA | 72.2 | IM | 6.8 | 17.6 |
| PA4412 | murG | UDP-N-acetylglucosamine–N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase | 37.8 | IM | < 2.0 | 5.1 |
| PA4413 | ftsW | Probable peptidoglycan glycosyltransferase FtsW | 43.8 | IM | < 2.0 | 2.3 |
| PA4414 | murD | UDP-N-acetylmuramoylalanine–D-glutamate ligase | 48.1 | C | 2.3 | < 2.0 |
| PA4417 | murE | UDP-N-acetylmuramoyl-L-alanyl-D-glutamate–2,6-diaminopimelate ligase | 51.3 | C | < 2.0 | 4.2 |
| PA4418 | ftsI (PBP3) | Peptidoglycan D,D-transpeptidase FtsI | 62.9 | IM | 59.4 | 5.5 |
| PA4450 | murA | UDP-N-acetylglucosamine-1-carboxyvinyltransferase | 44.6 | C | 2.5 | <2.0 |
| PA4700 | mrcB (PBP1b) | Penicillin-binding protein 1B | 85.5 | IM | 7.7 | 3.6 |
| PA4749 | glmM | Phosphoglucosamine mutase | 47.8 | C | 4.2 | < 2.0 |
| PA4947 | amiB | N-acetylmuramoyl-L-alanine amidase | 50.7 | OM | 3.9 | < 2.0 |
| PA5045 | mrcA (PBP1a) | Penicillin-binding protein 1A | 91.2 | IM | 3.0 | 6.0 |
| PA5485 | ampDh2 | AmpDh2 | 28.9 | OM | 4.1 | 7.0 |