Abstract
To evaluate the sensitivity and specificity of the direct specimen test with monoclonal antibody conjugated with fluorescein isothiocyanate (FITC), cervical smears from 700 Viennese prostitutes were investigated by immunofluorescence microscopy and compared with the results of cultures for Chlamydia trachomatis in McCoy cells. Inclusion bodies in cell culture and elementary bodies on the smear slide were counted and evaluated in four categories. Cultures were obtained from 12.6% (88) of all women investigated, whereas the direct specimen test gave positive results in 10.4% (73). The results indicate that in asymptomatic people with only few inclusion bodies and latent infections, culture on McCoy cells is the more adequate method of identifying Chlamydia trachomatis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arth C., Von Schmidt B., Grossman M., Schachter J. Chlamydial pneumonitis. J Pediatr. 1978 Sep;93(3):447–449. doi: 10.1016/s0022-3476(78)81155-x. [DOI] [PubMed] [Google Scholar]
- Dalaker K., Gjønnaess H., Kvile G., Urnes A., Anestad G., Bergan T. Chlamydia trachomatis as a cause of acute perihepatitis associated with pelvic inflammatory disease. Br J Vener Dis. 1981 Feb;57(1):41–43. doi: 10.1136/sti.57.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harrison H. R., Alexander E. R., Chiang W. T., Giddens W. E., Jr, Boyce J. T., Benjamin D., Gale J. L. Experimental nasopharyngitis and pneumonia caused by Chlamydia trachomatis in infant baboons: histopathologic comparison with a case in a human infant. J Infect Dis. 1979 Feb;139(2):141–146. doi: 10.1093/infdis/139.2.141. [DOI] [PubMed] [Google Scholar]
- Hobson D., Karayiannis P., Byng R. E., Rees E., Tait I. A., Davies J. A. Quantitative aspects of chlamydial infection of the cervix. Br J Vener Dis. 1980 Jun;56(3):156–162. doi: 10.1136/sti.56.3.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mallinson H., Arya O. P., Goddard A. D. Quantitative study of Chlamydia trachomatis in genital infection. Br J Vener Dis. 1982 Feb;58(1):36–39. doi: 10.1136/sti.58.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mösinger-Lundgren V., Hofmann H., Petzoldt D. Die Häufigkeit genitaler Chlamydien- und Mykoplasmen-Infektionen bei Frauen. Z Hautkr. 1984 Jan 1;59(1):43–47. [PubMed] [Google Scholar]
- Paavonen J., Valtonen V. V. Chlamydia trachomatis as a possible cause of peritonitis and perihepatitis in a young woman. Br J Vener Dis. 1980 Oct;56(5):341–343. doi: 10.1136/sti.56.5.341. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stamm W. E., Harrison H. R., Alexander E. R., Cles L. D., Spence M. R., Quinn T. C. Diagnosis of Chlamydia trachomatis infections by direct immunofluorescence staining of genital secretions. A multicenter trial. Ann Intern Med. 1984 Nov;101(5):638–641. doi: 10.7326/0003-4819-101-5-638. [DOI] [PubMed] [Google Scholar]
- Stary A., Gebhart W., Gross W., Kopp W., Söltz-Szöts J., Thurner J. Genitale Chlamydieninfektionen bei STD- (= Sexually transmitted diseases-) Risikopatientinnen. Wien Klin Wochenschr. 1982 Oct 1;94(18):484–487. [PubMed] [Google Scholar]
- Sweet R. L. Pelvic inflammatory disease: etiology, diagnosis, and treatment. Sex Transm Dis. 1981 Oct-Dec;8(4 Suppl):308–315. [PubMed] [Google Scholar]
- Treharne J. D., Ripa K. T., Mårdh P. A., Svensson L., Weström L., Darougar S. Antibodies to Chlamydia trachomatis in acute salpingitis. Br J Vener Dis. 1979 Feb;55(1):26–29. doi: 10.1136/sti.55.1.26. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weström L. Effect of acute pelvic inflammatory disease on fertility. Am J Obstet Gynecol. 1975 Mar 1;121(5):707–713. doi: 10.1016/0002-9378(75)90477-9. [DOI] [PubMed] [Google Scholar]