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. 2023 Mar 21;26(4):106462. doi: 10.1016/j.isci.2023.106462

Figure 3.

Figure 3

Pyruvate partially and 2-DG almost completely reverses the Crabtree effect in PTEC

(A–C) HK-2 cells were switched to assay media (Seahorse XF base medium with 5 mM HEPES) with indicated concentration of glucose along with pyruvate in concentration range of 0.5–3 mM 1 h prior the assay. Changes in OCR and ECAR were measured before and after sequential injections of oligomycin, FCCP, and rotenone/antimycin A. Data are represented as mean ± SEM.

(D and E) HK-2 cells were switched to assay media with no glucose or 5.5 mM glucose 1 h prior the assay and indicated concentrations of 2-DG were injected to cells following rate 3 through XF cartridge port A followed by injections of oligomycin, FCCP, and rotenone + antimycin A. Data are shown as mean ± SD from a representative experiment.

(F–H) Basal OCR, maximal OCR, and ECAR calculated as described in STAR Methods section. Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 compared to NG by one-way ANOVA followed by Dunnett’s post-hoc test for multiple comparisons.