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. 1986 Oct;62(5):321–328. doi: 10.1136/sti.62.5.321

Immunoblot analysis of antigens associated with Haemophilus ducreyi using serum from immunised rabbits.

J M Saunders, J D Folds
PMCID: PMC1011985  PMID: 3490427

Abstract

Sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting were used to characterise isolates of Haemophilus ducreyi. Isolates of H ducreyi were heterogeneous in protein composition, but isolates from single outbreaks appeared similar both in protein profiles and antigenic analysis. Rabbits immunised with H ducreyi responded with a vigorous humoral immune response in which multiple antigenic polypeptides were detected. The most prominent antigens had molecular masses of 67, 42, 22.5, and 20 kilodaltons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chapel T. A., Brown W. J., Jeffres C., Stewart J. A. How reliable is the morphological diagnosis of penile ulcerations? Sex Transm Dis. 1977 Oct-Dec;4(4):150–152. doi: 10.1097/00007435-197710000-00008. [DOI] [PubMed] [Google Scholar]
  2. Denys G. A., Chapel T. A., Jeffries C. D. An indirect fluorescent antibody technique for Haemophilus ducreyi. Health Lab Sci. 1978 Jul;15(3):128–132. [PubMed] [Google Scholar]
  3. Hammond G. W., Lian C. J., Wilt J. C., Ronald A. R. Comparison of specimen collection and laboratory techniques for isolation of Haemophilus ducreyi. J Clin Microbiol. 1978 Jan;7(1):39–43. doi: 10.1128/jcm.7.1.39-43.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hammond G. W., Slutchuk M., Scatliff J., Sherman E., Wilt J. C., Ronald A. R. Epidemiologic, clinical, laboratory, and therapeutic features of an urban outbreak of chancroid in North America. Rev Infect Dis. 1980 Nov-Dec;2(6):867–879. doi: 10.1093/clinids/2.6.867. [DOI] [PubMed] [Google Scholar]
  5. Hansen E. J., Loftus T. A. Monoclonal antibodies reactive with all strains of Haemophilus ducreyi. Infect Immun. 1984 Apr;44(1):196–198. doi: 10.1128/iai.44.1.196-198.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kinghorn G. R., Hafiz S., McEntegart M. G. Modified haemin-containing medium for isolation of Haemophilus ducreyi. Lancet. 1982 Feb 13;1(8268):393–394. doi: 10.1016/s0140-6736(82)91417-9. [DOI] [PubMed] [Google Scholar]
  7. Kinghorn G. R., Hafiz S., McEntegart M. G. Pathogenic microbial flora of genital ulcers in Sheffield with particular reference to herpes simplex virus and Haemophilus ducreyi. Br J Vener Dis. 1982 Dec;58(6):377–380. doi: 10.1136/sti.58.6.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Oberhofer T. R., Back A. E. Isolation and cultivation of Haemophilus ducreyi. J Clin Microbiol. 1982 Apr;15(4):625–629. doi: 10.1128/jcm.15.4.625-629.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Odumeru J. A., Ronald A. R., Albritton W. L. Characterization of cell proteins of Haemophilus ducreyi by polyacrylamide gel electrophoresis. J Infect Dis. 1983 Oct;148(4):710–714. doi: 10.1093/infdis/148.4.710. [DOI] [PubMed] [Google Scholar]
  11. Ronald A. R., Plummer F. A. Chancroid and Haemophilus ducreyi. Ann Intern Med. 1985 May;102(5):705–707. doi: 10.7326/0003-4819-102-5-705. [DOI] [PubMed] [Google Scholar]
  12. Saunders J. M., Folds J. D. Humoral response of the mouse to Treponema pallidum. Genitourin Med. 1985 Aug;61(4):221–229. doi: 10.1136/sti.61.4.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sottnek F. O., Biddle J. W., Kraus S. J., Weaver R. E., Stewart J. A. Isolation and identification of Haemophilus ducreyi in a clinical study. J Clin Microbiol. 1980 Aug;12(2):170–174. doi: 10.1128/jcm.12.2.170-174.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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