Fig. 4.

Increasing FOXO1 expression in HCC upregulated IRF-1 by binding to the promoter. A, B, The correlation between FOXO1 and IRF-1 mRNA expression in HCC samples. C, D, Expression of IRF-1 was detected in Hepa1-6 and Huh7 cells with different FOXO1 expression levels. E, The mechanic graph shows the predicted binding sites of FOXO1 located in the promoter of IRF-1; F, PCR products of ChIP performed on Hepa1-6 cells ran on a 1% agarose gel. RNA pol II group was the positive control, Negative IgG group and water group were the negative control, FOXO1 antibody enriched in the DNA promoters. G, IRF-1 was confirmed to be the promoter binding by FOXO1 by qPCR through specific primers, IgG was the negative control group. H, Luciferase reporter assays were performed to show the activity of IRF-1 promoter transfection (mean±SEM, *P<0.05).