Fig. 6.

IL-6/STAT3 was activated in the interaction between different FOXO1 HCC cell groups and induced macrophages. A, Quantification of migrated Hepa1-6 cells toward CM from mice PM by transwell assay; B, Proliferation assays were performed in Hepa1-6 cells treated with CM from PM induced by Lv-control-Hepa1-6, Lv-Foxo1-Hepa1-6, combined with IL-6 neutralizing antibody or not; C, p-STAT3, STAT3, Vimentin, N-cadherin expression were detected in Hepa1-6 cells treated with CM from Lv-control-Hepa1-6, Lv-FOXO1-Hepa1-6 induced macrophages combined with IL-6 neutralizing antibody or not by western blot. D, IHC staining of FOXO1-, P-STAT3-, and Vimentin was performed in different FOXO1 expression groups in human HCC samples. (Mean±SEM, *P<0.05).