Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | UAS-PGA | This paper | FLAG-tagged penicillin G acylase under UAS control | |
| Genetic reagent (D. melanogaster) | Actin5C-Gal4 | Bloomington Drosophila Stock Center | BDSC:25374 FLYB:FBti012 7834; RRID:BDSC_ 25374 |
FlyBase symbol: P{Act5C-GAL4-w}E1 |
| Genetic reagent (D. melanogaster) | Elav(C155)-Gal4 | Bloomington Drosophila Stock Center | BDSC:458 FLYB:FBti000 2575; RRID:BDSC_ 458 |
FlyBase symbol: P{GawB}elav [C155] |
| Genetic reagent (D. melanogaster) | Repo-Gal4 | Bloomington Drosophila Stock Center | BDSC:7415 FLYB:FBti001 8692 RRID:BDSC_ 7415 |
FlyBase symbol: P{GAL4}repo |
| Genetic reagent (D. melanogaster) | TH-Gal4 | Bloomington Drosophila Stock Center | BDSC:8848 FLYB:FBti007 2936 RRID:BDSC_ 8848 |
FlyBase symbol: P{ple-GAL4.F}3 |
| Biological sample (D. melanogaster) | Drosophila brain explants | This paper | Freshly isolated from various D. melanogaster genotypes | |
| Antibody | Anti-Biotin rabbit polyclonal | Bethyl Laboratories Inc. | Cat: A150109A | 1:1000 |
| Antibody | Anti-Brp (nc82) mouse monoclonal | DSHB | Cat: nc82 | 1:50 |
| Antibody | Anti-Synapsin (3C11) mouse monoclonal | DSHB | Cat: 3C11 | 1:500 |
| Antibody | Anti-Syntaxin (8C3) mouse monoclonal | DSHB | Cat: 8C3 | 1:500 |
| Antibody | Anti-Draper 8A1 mouse monoclonal | DSHB | Cat: 8A1 | 1:400 |
| Antibody | Anti-Draper 5D14 mouse monoclonal | DSHB | Cat: 5D14 | 1:400 |
| Antibody | Anti-Elav 9F8A9 mouse monoclonal | DSHB | Cat: 9F8A9 | 1:100 |
| Antibody | Anti-FLAG (M2) mouse monoclonal | Millipore Sigma | Cat: F1804 | 1:500 |
| Antibody | Anti-FLAG (L5) mouse monoclonal | Novus Biologicals | Cat: NBP1-06712 | 1:1000 |
| Antibody | Anti-Actin-HRP (AC15) mouse monoclonal | Millipore Sigma | Cat: A3854 | 1:2000 |
| Antibody | Anti-GAPDH (GA1R) mouse monoclonal | ThermoFisher | Cat:MA5-15738 | 1:10,000 |
| Antibody | Anti-TH mouse monoclonal | Immunostar | Cat: 22941 | 1:1000 |
| Antibody | Anti-Ubiquitin (P4D1) rabbit monoclonal | Cell Signaling Technology | Cat: 3936 | 1:1000 |
| Commercial assay or kit | Click-iT Plus OPP Alexa Fluor 488 Protein Synthesis Assay Kit | ThermoFisher | Cat: C10456 | |
| Chemical compound, drug | PhAc-OPP | This paper and reference (Barrett et al., 2016) | See reference (Barrett et al., 2016) for chemical synthesis details | |
| Chemical compound, drug | Desthiobiotin azide | Click Chemistry Tools | Cat: 50-210-7822 | |
| Chemical compound, drug | TBTA (Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine) | Millipore Sigma |
Cat:678937 | |
| Chemical compound, drug | Cu(I)Br | Millipore Sigma |
Cat: 61163 | |
| Chemical compound, drug | Neutravidin agarose | Thermo Scientific | Cat: P129204 | |
| Chemical compound, drug | Colloidal blue staining kit | ThermoFisher | Cat: LC6025 |