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. 1977 Jun;14(3):157–162. doi: 10.1136/jmg.14.3.157

Differentiation in human amniotic fluid cell cultures: I: Collagen production.

R E Priest, J H Priest, J F Moinuddin, A J Keyser
PMCID: PMC1013548  PMID: 881704

Abstract

The collagen produced by differentiated cells cultured from human amniotic fluid was characterized in two ways. By chain composition and by 4-hydroxyproline:3-hydroxyproline isomer ratio, the collagen synthesized by F-type (fibroblast) cells was indistinguishable from that made by cultured fetal dermal fibroblasts. The predominant cells in young amniotic fluid cultures, termed AF-type, produced collagen with a lower isomer ratio, resembling that of basement membrane collage. The chain composition, as determined by chromatography on carboxymethyl cellulose, varied for different cultures of the AF-type, but the major pattern was consistent with that of basement membrane collagen. On the basis of these characteristics, F cells are of fibroblast origin, whereas most AF cells are of a different origin either endothelial or epithelial. Other evidence (Megaw et al., 1977) suggests an epithelial origin for AF cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clark C. C., Tomichek E. A., Koszalka T. R., Minor R. R., Kefalides N. A. The embryonic rat parietal yolk sac. The role of the parietal endoderm in the biosynthesis of basement membrane collagen and glycoprotein in vitro. J Biol Chem. 1975 Jul 10;250(13):5259–5267. [PubMed] [Google Scholar]
  2. Grant M. E., Harwood R., Williams I. F. The biosynthesis of basement-membrane collagen by isolated rat glomeruli. Eur J Biochem. 1975 Jun;54(2):531–540. doi: 10.1111/j.1432-1033.1975.tb04166.x. [DOI] [PubMed] [Google Scholar]
  3. Grant M. E., Kefalides N. A., Prockop D. J. The biosynthesis of basement membrane collagen in embryonic chick lens. I. Delay between the synthesis of polypeptide chains and the secretion of collagen by matrix-free cells. J Biol Chem. 1972 Jun 10;247(11):3539–3544. [PubMed] [Google Scholar]
  4. Hoehn H., Bryant E. M., Karp L. E., Martin G. M. Cultivated cells from diagnostic amniocentesis in second trimester pregnancies. I. Clonal morphology and growth potential. Pediatr Res. 1974 Aug;8(8):746–754. doi: 10.1203/00006450-197408000-00003. [DOI] [PubMed] [Google Scholar]
  5. Hurych J., Macek M., Beniac F., Rezácová D. Biochemical characteristics of collagen produced by long term cultivated amniotic fluid cells. Hum Genet. 1976 Mar 12;31(3):335–340. doi: 10.1007/BF00270863. [DOI] [PubMed] [Google Scholar]
  6. Kefalides N. A., Cameron J. D., Tomichek E. A., Yanoff M. Biosynthesis of basement membrane collagen by rabbit corneal endothelium in vitro. J Biol Chem. 1976 Feb 10;251(3):730–733. [PubMed] [Google Scholar]
  7. Kefalides N. A. Isolation of a collagen from basement membranes containing three identical - chains. Biochem Biophys Res Commun. 1971 Oct 1;45(1):226–234. doi: 10.1016/0006-291x(71)90073-8. [DOI] [PubMed] [Google Scholar]
  8. Mayne R., Vail M. S., Miller E. J. Analysis of changes in collagen biosynthesis that occur when chick chondrocytes are grown in 5-bromo-2'-deoxyuridine. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4511–4515. doi: 10.1073/pnas.72.11.4511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Megaw J. M., Priest J. H., Priest R. E., Johnson L. D. Differentiation in human amniotic fluid cell cultures: II: Secretion of an epithelial basement membrane glycoprotein. J Med Genet. 1977 Jun;14(3):163–167. doi: 10.1136/jmg.14.3.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miller E. J. Isolation and characterization of a collagen from chick cartilage containing three identical alpha chains. Biochemistry. 1971 Apr 27;10(9):1652–1659. doi: 10.1021/bi00785a024. [DOI] [PubMed] [Google Scholar]
  11. PROCKOP D. J., EBERT P. S. A SIMPLE METHOD FOR DIFFERENTIAL ASSAY OF TRITIUM AND CARBON-14 IN WATER-SOLUBLE BIOLOGICAL MATERIALS. Anal Biochem. 1963 Sep;6:263–271. doi: 10.1016/0003-2697(63)90134-9. [DOI] [PubMed] [Google Scholar]
  12. Pinnell S. R., Krane S. M., Kenzora J. E., Glimcher M. J. A heritable disorder of connective tissue. Hydroxylysine-deficient collagen disease. N Engl J Med. 1972 May 11;286(19):1013–1020. doi: 10.1056/NEJM197205112861901. [DOI] [PubMed] [Google Scholar]
  13. Priest R. E., Moinuddin J. F., Priest J. H. Letter: Collagen of Marfan syndrome is abnormally soluble. Nature. 1973 Oct 5;245(5423):264–266. doi: 10.1038/245264a0. [DOI] [PubMed] [Google Scholar]
  14. Schmid W. The prenatal diagnosis of chromosome anomalies. Triangle. 1972;11(3):91–102. [PubMed] [Google Scholar]
  15. Sutherland G. R., Bauld R., Bain A. D. Observations on human amniotic fluid cell strains in serial culture. J Med Genet. 1974 Jun;11(2):190–195. doi: 10.1136/jmg.11.2.190. [DOI] [PMC free article] [PubMed] [Google Scholar]

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