Table 1.
KIE and non-isotopic kinetic measurements from L8 vs. L8S8 rubiscos.
| Strain | Rubisco | KIE (‰) | VC (s−1) | KC (μM) | SC/O | VO (s−1) | KO (μM) |
|---|---|---|---|---|---|---|---|
| Synechococcus elongatus PCC6301 | L8S8 | 22.42 ± 2.37 | 14.3 ± 0.71 | 235 ± 20.0 | 56.1 ± 1.3 | 1.10 | 983 ± 81 |
| Candidatus Promineofilum breve | L8 | 16.25 ± 1.36 | 2.23 ± 0.04 | 22.2 ± 9.7 | 36.1 ± 0.9 | 1.11 | 401 ± 115 |
KIEs were measured in this study using the substrate depletion method [25,35,36,37]; see Methods for more detail. Non-isotopic kinetic measurements are from [30]. VC and VO indicate maximum carboxylation and oxygenation rates under substrate-saturated conditions, respectively; KC and KO are Michaelis constants for the carboxylation and oxygenation reactions, respectively; SC/O indicates specificity, a unitless measure of the relative preference for CO2 over O2 and is calculated as SC/O = (VC/KC)/(VO/KO). Uncertainties on non-isotopic kinetics reflect mean ± s.e.m. from multiple experiments; see [30] for more detail. Error on KIEs reflect mean ± s.d. from model fitting uncertainty from one experiment; see Methods and Supplemental for more detail.