Table 1.
Screening of the neutralization activity of NBCoV compounds against NL4-3ΔEnv-NanoLuc/SARS-CoV-2 (USA-WA1/2020) pseudovirus (IC50), toxicity evaluation (CC50), and selectivity indexes (SI) in two cell lines.
| Compounds | Structure | 293T/ACE2 Cells | A549/ACE2/TMPRSS2 Cells | ||||
|---|---|---|---|---|---|---|---|
| IC50 (nM) a | CC50 (µM) a | SI | IC50 (nM) a | CC50 (µM) a | SI | ||
| NBCoV35 |
|
198 ± 9 | ~50 | 253 | 126 ± 3 | ~50 | 397 |
| NBCoV36 |
|
330 ± 18 | 43 ± 0.9 | 130 | 227 ± 18 | >50 | >220 |
| NBCoV37 |
|
66 ± 2 | 42.5 ± 2 | 644 | 145 ± 12 | ~50 | 345 |
| NBCoV62 |
|
>10,000 | >50 | ND | >10,000 | >50 | ND |
| NBCoV63 |
|
80 ± 7 | >50 | >625 | 55 ± 3 | ~50 | 909 |
| NBCoV65 |
|
>10,000 | >50 | ND | >10,000 | >50 | ND |
| NBCoV66 |
|
>10,000 | >50 | ND | >10,000 | >50 | ND |
| NBCoV68 |
|
3900 ± 100 | >50 | >13 | 2300 ± 70 | >50 | >22 |
| NBCoV69 |
|
2550 ± 105 | >50 | >20 | 3000 ± 400 | >50 | >17 |
| NBCoV70 |
|
9500 ± 1000 | >50 | >5 | >10,000 | >50 | ND |
| NBCoV71 |
|
>10,000 | 48 ± 2 | ND | >10,000 | 40 ± 2 | ND |
| NBCoV72 |
|
>10,000 | >50 | ND | >10,000 | >50 | ND |
| NBCoV73 |
|
>10,000 | >50 | ND | >10,000 | >50 | ND |
| NBCoV74 |
|
>10,000 | >50 | ND | >10,000 | >50 | ND |
| NBCoV75 |
|
5200 ± 600 | >50 | >10 | 3300 ± 700 | >50 | >15 |
| NBCoV76 |
|
>10,000 | >50 | ND | >10,000 | >50 | ND |
| NBCoV77 |
|
>10,000 | >50 | ND | >10,000 | >50 | ND |
| NBCoV78 |
|
>10,000 | >50 | ND | 8100 ± 200 | >50 | >6 |
| NBCoV79 |
|
>10,000 | >50 | ND | >10,000 | >50 | ND |
| NBCoV80 |
|
>10,000 | >50 | ND | >10,000 | >50 | ND |
| NBCoV81 |
|
387 ± 64 | >50 | >129 | 459 ± 23 | >50 | >109 |
| NBCoV82 |
|
>10,000 | >50 | ND | >10,000 | >50 | ND |
a The reported IC50 and CC50 values represent the means ± standard deviations (n = 3). ND = Not Determined.