Table 2.
Antiviral activity of the NBCoV small molecules in the single-cycle assay in two cell lines against pseudovirus NL4-3ΔEnv-NanoLuc/SARS-CoV-2 mutant (D614G) and its variants of concern (VOC) (IC50).
| Compounds | SARS-CoV-2 IC50 (nM) a |
||||
|---|---|---|---|---|---|
| USA-WA1/2020 D614G |
Variants of Concern (VOC) | ||||
| Delta (B.1.617.2) | Omicron (B.1.1.529/BA.1) | Omicron (BA.4/BA.5) | Brazil (Gamma: K417T/E484K/N501Y) | ||
| 293T/ACE2 Cells | |||||
| NBCoV35 | 195 ± 10 | 335 ± 5 | 173 ± 30 | 94 ± 6 | 506 ± 44 |
| NBCoV36 | 430 ± 31 | 802 ± 143 | 368 ± 90 | 181 ± 12 | 1183 ± 58 |
| NBCoV37 | 157 ± 1.5 | 267 ± 23 | 135 ± 3 | 82 ± 8 | 260 ± 52 |
| NBCoV63 | 40.8 ± 1.2 | 84 ± 0.5 | 96 ± 11 | 34 ± 1 | 77.5 ± 17.3 |
| A549/ACE2/TMPRSS2 Cells | |||||
| NBCoV35 | 266 ± 15 | 236 ± 6 | 201 ± 38 | 170 ± 13 | 292 ± 23 |
| NBCoV36 | 150 ± 2 | 332 ± 26 | 129 ± 2 | 310 ± 21 | 149 ± 26 |
| NBCoV37 | 311 ± 33 | 130 ± 35 | 42.5 ± 1 | 215 ± 10 | 73 ± 14 |
| NBCoV63 | 42.5 ± 1 | 105 ± 1 | 60 ± 3 | 26 ± 0.5 | 45.5 ± 2 |
a The reported IC50 values represent the means ± standard deviations (n = 3).