Table 4.
Antiviral activity of the NBCoV small molecules in the single-cycle assay in two cell lines against pseudovirus NL4-3ΔEnv-NanoLuc/MERS-CoV (IC50), toxicity (CC50), and SI.
| Compound | MERS-CoV | |||||
|---|---|---|---|---|---|---|
| Caco-2 Cells | MRC-5 Cells | |||||
| IC50 (nM) a | CC50 (µM) a | SI | IC50 (nM) a | CC50 (µM) a | SI | |
| NBCoV35 | 472 ± 43 | >50 | >106 | 715 ± 43 | >50 | >70 |
| NBCoV36 | 428 ± 34 | >50 | >117 | 837 ± 38 | >50 | >60 |
| NBCoV37 | 161 ± 53 | >50 | >311 | 229 ± 20 | 48 ± 2 | 210 |
| NBCoV63 | 75 ± 25 | >50 | >667 | 102 ± 1 | >50 | >490 |
a The reported IC50 and CC50 values represent the means ± standard deviations (n = 3).