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. 2023 Mar 31;26(4):106545. doi: 10.1016/j.isci.2023.106545

Figure 4.

Figure 4

Oxytocin attenuates AβO-induced microglial activation in vitro

Microglia were immunopanned from P18 rat pups, kept in culture for 5 DIV and treated with oxytocin (OXT, 10 nM) and/or AβOs (500 nM) for 3h.

(A) Representative Iba1 immunocytochemistry images.

(B), Iba1 immunoreactivity; Veh versus AβOs, p = 0.0077; AβOs versus OXT AβOs, p = 0.0171.

(C) CD68 immunoreactivity; Veh versus AβOs, p = 0.0323; AβOs versus OXT AβOs, p = 0.0167.

(D) Number of primary microglial processes per cell; Veh versus AβOs, p = 0.0009; AβOs versus OXT AβOs, p = 0.0190. N = 12–19 independent cultures. (B) Friedman test and (C and D) repeated measures ANOVA followed by Dunnett’s post-hoc test. Bars represent means ± SEM. Symbols represent mean values from independent microglial cultures. Scale bar: 50 μm.