Abstract
The use of chromosome in situ suppression hybridisation with whole chromosome libraries has previously been reported by various research laboratories to be an effective method of identifying specific human chromosomal material. As a clinical cytogenetic service laboratory we have used the technique as a complement to diagnosis by classical chromosome banding. In three examples of structural rearrangements the potential use of the 'chromosome painting' method is assessed for its ability to enhance the routine cytogenetic service currently available.
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- Arnoldus E. P., Wiegant J., Noordermeer I. A., Wessels J. W., Beverstock G. C., Grosveld G. C., van der Ploeg M., Raap A. K. Detection of the Philadelphia chromosome in interphase nuclei. Cytogenet Cell Genet. 1990;54(3-4):108–111. doi: 10.1159/000132972. [DOI] [PubMed] [Google Scholar]
- Cremer T., Lichter P., Borden J., Ward D. C., Manuelidis L. Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes. Hum Genet. 1988 Nov;80(3):235–246. doi: 10.1007/BF01790091. [DOI] [PubMed] [Google Scholar]
- Dietz H. C., Pyeritz R. E., Hall B. D., Cadle R. G., Hamosh A., Schwartz J., Meyers D. A., Francomano C. A. The Marfan syndrome locus: confirmation of assignment to chromosome 15 and identification of tightly linked markers at 15q15-q21.3. Genomics. 1991 Feb;9(2):355–361. doi: 10.1016/0888-7543(91)90264-f. [DOI] [PubMed] [Google Scholar]
- Jauch A., Daumer C., Lichter P., Murken J., Schroeder-Kurth T., Cremer T. Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics. Hum Genet. 1990 Jul;85(2):145–150. doi: 10.1007/BF00193186. [DOI] [PubMed] [Google Scholar]
- Lichter P., Cremer T., Borden J., Manuelidis L., Ward D. C. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet. 1988 Nov;80(3):224–234. doi: 10.1007/BF01790090. [DOI] [PubMed] [Google Scholar]
- Lichter P., Cremer T., Tang C. J., Watkins P. C., Manuelidis L., Ward D. C. Rapid detection of human chromosome 21 aberrations by in situ hybridization. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9664–9668. doi: 10.1073/pnas.85.24.9664. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pinkel D., Landegent J., Collins C., Fuscoe J., Segraves R., Lucas J., Gray J. Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9138–9142. doi: 10.1073/pnas.85.23.9138. [DOI] [PMC free article] [PubMed] [Google Scholar]