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. 2023 Apr 20;12:e83209. doi: 10.7554/eLife.83209

Figure 3. Loss of YAP limited cell proliferation and promoted valve shaping.

(A-B). Cushion explants were cultured under CS (compressive stress), CS +VP (compressive stress +YAP inhibitor), U+VP (unloaded +YAP inhibitor) conditions for 24 hr. (C). After 24-hr-culture, explants were stained for YAP (green) and proliferation marker pHH3 (red, arrows), or (D). YAP (green) and VE-Cadherin (red). (E). Circularity of explants cultured under different stress conditions, which describes how close a valve is to a perfect sphere. (F). Percentages of cells expressing pHH3 under different culture conditions. (G). Average intensities of VE-Cad expression under different culture conditions, the intensities are normalized to maximum intensity. Data are presented by mean ± SEM, n=15 explant valves from eight embryos, *p<0.05, two-tailed student t-tests.

Figure 3—source data 1. Data used to generate Figure 3E, F and G.

Figure 3.

Figure 3—figure supplement 1. Verteporfin and PY-60 treatments.

Figure 3—figure supplement 1.

Live and dead staining of (A). verteporfin and (B). PY-60 treated cushion explants. (C). Expression ratios of nuclear vs. cytoplasmic YAP show the effective inhibition of YAP by verteporfin. (D). Survival rate was not affected by the treatments.