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. Author manuscript; available in PMC: 2024 Apr 6.
Published in final edited form as: Structure. 2023 Mar 3;31(4):385–394.e4. doi: 10.1016/j.str.2023.02.005

Figure 1. Structure determination of the T-pilus.

Figure 1.

(A) SDS-PAGE of purified VirB2 protein stained with Coomassie (lane 2). Mature VirB2 has a predicted MW of 7.2 kDa. Precision plus molecular weight marker was used (lane 1). (B) Cryo-electron micrograph of intact purified T-pilus (C) Representative 2D-class averages from Relion with a 300 pixel box size. (D) Bottom view of the filament model; coloured by individual VirB2 molecules. (E) Side-view of the filament. The filament is built up by five parallel helices, each of these have a unique colour. The top of the represented filament is towards the tip (as determined before52).