Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-VirB2 | Mossey et al., 2010.33 | N/A |
| Bacterial and virus strains | ||
| A. tumefaciens NT1REB(pJK270) | Chesnokova et al., 1997.28 | N/A |
| A. tumefaciens PC1002 | Berger and Christie, 1994.53 | N/A |
| Biological samples | ||
| Kalanchoe daigremontiana | Prof. Eugene W. Nester, Dept. of Microbiology & Immunology, University of Seattle, WA. | N/A |
| Critical commercial assays | ||
| QuickChangee II site-directed mutagenesis kit | Agilent Technologies | Cat#200523 |
| Deposited data | ||
| Cryo-EM map of the T-pilus | This study | EMD-28957 |
| Structure model of the T-pilus | This study | PDB ID: 8FAI |
| Oligonucleotides | ||
| Primers: see VirB2 mutagenesis section | Integrated DNA Technologies inc., Coralville, IA. | N/A |
| Recombinant DNA | ||
| pAD1412 | Mossey et al., 2010.33 | N/A |
| pAD1891 | Mossey et al., 2010.33 | N/A |
| pAD2161 (B2wt) | This study | N/A |
| pAD2162 (B2R91E) | This study | N/A |
| pAD2163 (B2R91A) | This study | N/A |
| pAD2164 (B2S93A) | This study | N/A |
| Software and algorithms | ||
| R version 4.1.2 | R Core Team, 2013.66 | http://www.r-project.org |
| Serial-EM | Mastronarde 2005.54 | http://bio3d.colorado.edu/SerialEM |
| Relion 4.0beta2 | Scheres, 2012.32 | https://relion.readthedocs.io |
| Motioncorr2 | Zheng et al., 2017.55 | https://relion.readthedocs.io |
| CTFFIND-4.1 | Rohou and Grigorieff, 2015.56 | https://grigoriefflab.umassmed.edu/ |
| SWISS-MODEL | Guex et al., 2009.57 | https://swissmodel.expasy.org |
| Rosetta3 | Leaver-Fay et al., 2011.58 | https://www.rosettacommons.org |
| Coot | Emsley et al., 2010.59 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| ChimeraX | Goddard et al., 2018.60 | https://www.cgl.ucsf.edu/chimerax/ |
| Phenix | Adams et al., 2011.62 | https://phenix-online.org |
| NAMD | Philips et al., 2020.63 | http://www.ks.uiuc.edu/Research/namd/ |