Figure 3: Differential regulation of spine density and morphology by amyloid on tuft branches from B6 and PWK APP/PS1 mice.

(A) Tuft dendritic lengths (left) and branch points (right). Individual data points represent each reconstructed neuron (n=3–5/mouse); error bars are ± SD; asterisks denote post-hoc (p<0.05) after two-way ANOVA and corrections for multiple comparisons.

(B) Example deconvolved confocal image of an EGFP+ tuft branch segment. Spine density was acquired across dendrites (left) and individual spines measured for maximum head diameter (right).

(C) Tuft branch spine densities across genotype/treatment groups. Data points represent individual branches (n=10–15/mouse); error bars are ± SD; asterisks denote comparisons (p<0.05) identified between PLX5622 and control diet groups after two-way ANOVA and corrections for multiple comparisons.

(D) Spine head diameter cumulative distributions from B6 (left) and PWK (right). K-S tests were used to evaluate statistical differences (see Table S5).

Statistical analyses performed on B6 and PWK separately. For (A) and (C) *adjusted p<0.05 Bonferroni post-hoc tests (Table S5).