KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
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| E. coli Rosetta 2(DE3)pLysS | Millipore Sigma | Cat# 71401–3 |
| E. coli BL21-AI | Thermo Fisher Scientific | Cat# C607003 |
| E. coli NEB Stable | New England Biolabs | Cat# C3040H |
| Dolichospermum flos-aquae strain 1403/13F | SAMS LIMITED CCAP | Cat# 1403/13F |
| Halobacterium sp. NRC-1, Living, Plate | Carolina Biological Supply | Cat# 154801 |
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| Chemicals, peptides, and recombinant proteins | ||
|
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| Q5 High-Fidelity DNA Polymerase | New England Biolabs | Cat# M0491L |
| NEBuilder HiFi DNA Assembly Master Mix | New England Biolabs | Cat# E2621L |
| LB Broth (Lennox) | Sigma-Aldrich | Cat# L3022–1KG |
| Kanamycin sulfate | Sigma-Aldrich | Cat# K1377–25G |
| D-(+)-Glucose | Sigma-Aldrich | Cat# G7021–5KG |
| SoluLyse™ Bacterial Protein Extraction Reagent | Genlantis | Cat# L200500 |
| Cyanobacteria BG-11 Freshwater Solution | Sigma-Aldrich | Cat# C3061 |
| 20x PBS, Sterile | Teknova | Cat# P1225 |
| Lysozyme from chicken egg white | Sigma-Aldrich | Cat# L6876 |
| IPTG, Isopropyl β D 1 thiogalactopyranoside | Teknova | Cat# I3305 |
| Chloramphenicol | Sigma-Aldrich | Cat# C0378 |
| Ampicillin sodium salt | Sigma-Aldrich | Cat# A0166 |
| Tris-HCl 1 M, pH 7.5 | Teknova | Cat# T1075 |
| Sodium chloride (NaCl) | Sigma-Aldrich | Cat# S7653 |
| Magnesium sulfate heptahydrate | Sigma-Aldrich | Cat# 230391 |
| Casein hydrolysate | Sigma-Aldrich | Cat# 22090 |
| Gibco™ Bacto™ Yeast Extract | Gibco | Cat# 212750 |
| Sodium nitrate | Amresco | Cat# 0598 |
| Potassium phosphate monobasic | Sigma-Aldrich | Cat# P5655 |
| Sodium metasilicate nonahydrate | Sigma-Aldrich | Cat# S4392 |
| Sodium carbonate | Sigma-Aldrich | Cat# 71345 |
| Sodium bicarbonate | Sigma-Aldrich | Cat# S5761 |
| Citric acid | BDH Chemicals | Cat# BDH7397 |
| EDTA, disodium salt dihydrate | Alfa Aesar | Cat# A15161 |
| Ferric ammonium citrate | Amresco | Cat# 0846 |
| Urea | Sigma-Aldrich | Cat# 51456 |
| Trisodium citrate dihydrate | Sigma-Aldrich | Cat# S1804 |
| Potassium chloride | Sigma-Aldrich | Cat# 746436 |
| D-Sorbitol | Sigma-Aldrich | Cat# S6021 |
| Formic Acid, LC/MS Grade | Fisher Scientific | Cat# A11750 |
| Acetonitrile, LC/MS Grade | Fisher Scientific | Cat# A9554 |
| Water, LC/MS Grade | Fisher Scientific | Cat# W64 |
| Trypsin, TPCK Treated | ThermoFisher Scientific | Cat# 20233 |
| DSSO, crosslinker | ThermoFisher Scientific | Cat# A33545 |
| BS3, crosslinker | ThermoFisher Scientific | Cat# A39266 |
|
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| Deposited data | ||
|
| ||
| Cryo-electron tomogram for Ana GV (Figures 1 and 2A) | This study | EMD-29922 |
| Cryo-electron tomogram Mega GV (Figure S2A) | This study | EMD-29925 |
| Cryo-electron tomogram Halo GV, p-vac (Figure S9B) | This study | EMD-29924 |
| Cryo-electron tomogram Halo GV, c-vac (Figure S9C) | This study | EMD-29923 |
| Subtomogram average of the native Ana GV shell | This study | EMD-29921 |
| Subtomogram average of AnaS GV shell | This study | EMD-29916 |
| Integrative model of Ana GvpA/GvpC | This study | PDB 8GBS |
| XLMS data | This study | PXD038631 |
| Atomic model of GvpA2 | Huber et al.24 | PDB 7R1C |
|
| ||
| Oligonucleotides | ||
|
| ||
| Primers for Gibson assembly | Integrated DNA Technologies | N/A |
| Mutagenic oligo pool | Twist Bioscience | N/A |
|
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| Recombinant DNA | ||
|
| ||
| pST39 plasmid containing pNL29 Mega GV gene cluster | Addgene | Cat# 91696 |
|
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| Software and algorithms | ||
|
| ||
| SnapGene | SnapGene | https://www.snapgene.com/ |
| MATLAB | Mathworks | https://matlab.mathworks.com/ |
| Abaqus | Dassault Systmes | https://www.3ds.com/products-services/simulia/products/abaqus/ |
| SerialEM | Mastronarde55 | https://bio3d.colorado.edu/SerialEM/ |
| Warp | Tegunov and Cramer56 | http://www.warpem.com/warp/ |
| Dynamo | Castaño-Díez et al.57 | https://wiki.dynamo.biozentrum.unibas.ch/w/index.php/Main_Page |
| RELION | Zivanov et al.58 | https://relion.readthedocs.io/en/release-3.1/ |
| M | Tegunov et al.53 | http://www.warpem.com/warp/ |
| IMOD | Kremer et al.59 | https://bio3d.colorado.edu/imod/ |
| UCSF Chimera | Pettersen et al.60 | https://www.cgl.ucsf.edu/chimera/index.html |
| UCSF ChimeraX | Goddard et al.61 | https://www.rbvi.ucsf.edu/chimerax/ |
| Phenix | Adams et al.62 | https://phenix-online.org/ |
| Coot | Emsley et al.63 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| 3DFSC | Tan et al.64 | https://github.com/nysbc/Anisotropy |
| dynamo2m | Burt el al.65 | https://github.com/alisterburt/dynamo2m |
| autoalign_dynamo | Burt el al.65 | https://github.com/alisterburt/autoalign_dynamo |
| Xcalibur | ThermoFisher Scientific | https://www.thermofisher.com/us/en/home.html |
| Proteome Discoverer | ThermoFisher Scientific | https://www.thermofisher.com/us/en/home.html |
| XlinkX node for Proteome Discoverer | ThermoFisher Scientific | https://www.thermofisher.com/us/en/home.html |
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| Other | ||
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| C-flat Holey Carbon R2/2 300 Mesh | EMS | Cat# CF-223C-100 |
| Formvar/Carbon 200 mesh, Copper | Ted Pella | Cat# 01801 |
| Vitrobot Mark IV | FEI, Thermo Fisher Scientific | https://www.thermofisher.com/us/en/home/electron-microscopy/products/sample-preparation-equipment-em/vitrobot-system.html |
| Gatan K3 summit camera with post-column energy filter | Gatan | https://www.gatan.com/products/tem-imaging-spectroscopy/k3-cameras |
| 300 kV Titan Krios microscope | FEI, Thermo Fisher Scientific | https://www.thermofisher.com/us/en/home/electron-microscopy/products/transmissionelectron-microscopes.html |
| Tecnai T12 120 kV | FEI, Thermo Fisher Scientific | Out-of-production |
| EconoSpin 96-Well Filter Plate | Epoch Life Sciences | Cat# 2020–001 |
| Shaker incubator with cooling, CO2 control and illumination | Infors HT | Multitron II |
| Benchtop microcentrifuge | Eppendorf | Cat# 5430R |
| UltraCruz Filter Flask, PES, 0.22 | Santa Cruz Biotechnology | Cat# sc-359029 |
| 1000 mL Filter System, PES Filter Material, 0.22 μm | CELLTREAT | Cat# 229708 |
| EMD Millipore™ Steritop Threaded Bottle Top Filter | EMD Millipore | Cat# S2GPT05RE |
| STERICUP-GP500 | EMD Millipore | Cat# S2GPU10RE |
| NanoDrop 2000C series | Thermo Scientifc | Cat# ND-2000C |
| 1-cm-path-length quartz cuvette | Hellma Analytics | Cat# 176.700-QS |
| 18G BLUNT NEEDLE 1.5IN STERILE | VWR International | Cat# 89134–020 |
| 96 well filter plate | Bio Basic | SD5006 |
| Square Petri Dish with Grid | VWR | 60872–310 |
| S-Trap micro spin column | PROTIFI | Cat# C02-micro-80 |
| Aurora UHPLC Column, 25 cm × 75 μm, 1.6 μm C18 | Ion Opticks | Cat# AUR2–25075C18A-CSI |
| Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer | ThermoFisher Scientific | N/A |
| Easy-nLC 1200 | ThermoFisher Scientific | LC140 |