In the published article, there was an error in Figure 5 , panel A as published. The mistake was to show a statistically significant difference in the spleen, as indicated by an asterisk in panel A, top left. The correct panel A doesn’t show a statistically significant difference in the spleen, but only in the LNs, in agreement with the text of the result section, as in the sentence “There was a trend of higher gag-specific frequency when boost was performed at d100 post-prime as compared to boost at d30 in all organs, that reached statistical significance in LNs ( Figure 5A ).” The corrected Figure 5 and its caption appear below.
Figure 5.
Analysis of gag-specific CD8 T cell frequency, TCM-phenotype and in vivo killing activity at d45 post-boost. Female BALB/c mice were primed as in Figure 1 at d0. One set of primed mice was boosted with MVA-gag at d30 post-prime, and another at d100 post-prime. For each set, analysis was performed at d45 post-boost. (A, B). Frequency of gag-specific CD8 T cells (A) and percentage of TCM among gag-specific CD8 T cells (B) in spleen, LNs, BM and blood of primed/boosted mice. (C, D). Primed/boosted and untreated control mice were injected i.v. with a 1:1 mixture of gag-pulsed CFSEhigh cells and unpulsed CFSElow syngeneic spleen cells (approximately 10x10^6 cells each). After 3 hours, the percentages of CFSEhigh and CFSElow cells were measured in spleen, LNs and BM, and the percentage of gag-specific killing was determined. Examples of CFSE histograms (C) and summary of results (D). (A, B) summarize results of 5 independent prime/boost experiments with a total of 60 mice, including control untreated mice (note that at each time point, 3 untreated mice were examined as a control; results were similar to those of untreated control mice shown in Figures 2A, B). Each symbol represents a pool of 3 mice. (C, D) summarize results of 4 independent prime/boost experiments with a total of 36 mice, including control untreated mice (see example in panel C). In (C), numbers represent percentages of cells in the indicated regions. In (D), each symbol represents a single mouse. Statistical analysis was performed by either Student t test, after checking that distribution was normal by Shapiro-Wilk test, or Mann-Whitney test. Statistically significant differences are indicated (*P ≤ 0.05).
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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