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. 1985 Aug;40(8):587–591. doi: 10.1136/thx.40.8.587

Lung angiotensin converting enzyme activity in chronically hypoxic rats.

J M Kay, P M Keane, K L Suyama, D Gauthier
PMCID: PMC1020596  PMID: 2994249

Abstract

A study was carried out to test the hypothesis that the reduced lung angiotensin converting enzyme (ACE) activity which occurs in chronic hypoxia is related to the development of pulmonary hypertension rather than to hypoxia per se. Right ventricular mean systolic pressure (Prvs, mm Hg) and ACE activity (nmol/mg protein/min) in lung tissue homogenates were measured in seven groups of four rats placed in a hypobaric chamber (380 mm Hg; 51 kPa) for two to 24 days. Identical measurements were made on 11 groups of four rats, which were placed in the chamber for 24 days and then allowed to recover in room air for one to 153 days. After two days of hypoxia the mean Prvs (25.5 (SD 3.7] and the mean lung ACE activity (56 (4.6] did not differ significantly from control values. Exposure to hypoxia for four to 24 days caused a progressive increase in mean Prvs to 44.4 (5.9) and a progressive reduction in mean lung ACE activity to 34 (4.0). During recovery lung ACE activity increased and Prvs decreased, so that normal values were achieved by 15 and 56 days respectively. Decreased lung ACE activity may be related to haemodynamic factors associated with pulmonary hypertension rather than to hypoxia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beutler E., West C. Sex- and age-related differences in activity of angiotensin-converting enzyme in serum. Clin Chem. 1983 Aug;29(8):1570–1570. [PubMed] [Google Scholar]
  2. Caldwell R. W., Blatteis C. M. Effect of chronic hypoxia on angiotensin-induced pulmonary vasoconstriction and converting enzyme activity in the rat. Proc Soc Exp Biol Med. 1983 Mar;172(3):346–350. doi: 10.3181/00379727-172-41568. [DOI] [PubMed] [Google Scholar]
  3. Cushman D. W., Cheung H. S. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem Pharmacol. 1971 Jul;20(7):1637–1648. doi: 10.1016/0006-2952(71)90292-9. [DOI] [PubMed] [Google Scholar]
  4. Davidson D., Stalcup S. A. Systemic circulatory adjustments to acute hypoxia and reoxygenation in unanesthetized sheep. Role of renin, angiotensin II, and catecholamine interactions. J Clin Invest. 1984 Feb;73(2):317–328. doi: 10.1172/JCI111216. [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]
  5. Kay J. M. Effect of intermittent normoxia on chronic hypoxic pulmonary hypertension, right ventricular hypertrophy, and polycythemia in rats. Am Rev Respir Dis. 1980 Jun;121(6):993–1001. doi: 10.1164/arrd.1980.121.6.993. [DOI] [PubMed] [Google Scholar]
  6. Kay J. M., Keane P. M., Suyama K. L., Gauthier D. Angiotensin converting enzyme activity and evolution of pulmonary vascular disease in rats with monocrotaline pulmonary hypertension. Thorax. 1982 Feb;37(2):88–96. doi: 10.1136/thx.37.2.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Keane P. M., Kay J. M., Suyama K. L., Gauthier D., Andrew K. Lung angiotensin converting enzyme activity in rats with pulmonary hypertension. Thorax. 1982 Mar;37(3):198–204. doi: 10.1136/thx.37.3.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Leuenberger P. J., Stalcup S. A., Mellins R. B., Greenbaum L. M., Turino G. M. Decrease in angiotensin I conversion by acute hypoxia in dogs. Proc Soc Exp Biol Med. 1978 Sep;158(4):586–589. doi: 10.3181/00379727-158-40252. [DOI] [PubMed] [Google Scholar]
  9. Lieberman J. Elevation of serum angiotensin-converting-enzyme (ACE) level in sarcoidosis. Am J Med. 1975 Sep;59(3):365–372. doi: 10.1016/0002-9343(75)90395-2. [DOI] [PubMed] [Google Scholar]
  10. Meyrick B., Reid L. The effect of continued hypoxia on rat pulmonary arterial circulation. An ultrastructural study. Lab Invest. 1978 Feb;38(2):188–200. [PubMed] [Google Scholar]
  11. Oparil S., Low J., Koerner T. J. Altered angiotensin I conversion in pulmonary disease. Clin Sci Mol Med. 1976 Dec;51(6):537–543. doi: 10.1042/cs0510537. [DOI] [PubMed] [Google Scholar]
  12. Pitt B. R., Lister G. Pulmonary metabolic function in the awake lamb: effect of development and hypoxia. J Appl Physiol Respir Environ Exerc Physiol. 1983 Aug;55(2):383–391. doi: 10.1152/jappl.1983.55.2.383. [DOI] [PubMed] [Google Scholar]
  13. Stalcup S. A., Lipset J. S., Legant P. M., Leuenberger P. J., Mellins R. B. Inhibition of converting enzyme activity by acute hypoxia in dogs. J Appl Physiol Respir Environ Exerc Physiol. 1979 Feb;46(2):227–234. doi: 10.1152/jappl.1979.46.2.227. [DOI] [PubMed] [Google Scholar]
  14. Stalcup S. A., Lipset J. S., Woan J. M., Leuenberger P., Mellins R. B. Inhibition of angiotensin converting enzyme activity in cultured endothelial cells by hypoxia. J Clin Invest. 1979 May;63(5):966–976. doi: 10.1172/JCI109397. [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]
  15. Szidon P., Bairey N., Oparil S. Effect of acute hypoxia on the pulmonary conversion of angiotensin I to angiotensin II in dogs. Circ Res. 1980 Feb;46(2):221–226. doi: 10.1161/01.res.46.2.221. [DOI] [PubMed] [Google Scholar]
  16. Szidon P., Oparil S., Osikowicz G., Booyse F. M. Effect of hypoxia on the conversion of angiotensin I to II in cultured porcine pulmonary endothelial cells. Biochem Pharmacol. 1983 Apr 1;32(7):1201–1205. doi: 10.1016/0006-2952(83)90272-1. [DOI] [PubMed] [Google Scholar]

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