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Differentiation of NPC to NPC-iN requires the presence of ascorbate in the medium. Representative immunofluorescence images of cells that were maintained in A, C, E) differentiation medium without added ascorbate (-Asc) or with B, D, F) 0.1 μM ascorbate (+Asc). Staining was performed with mAb 82E1 (for the N-terminal of β-CTF/Αβ, green), mAb AM (for HS-anMan, green), mAb SYNfil (green), pAb anti-TH (red), and DAPI (for nuclei, blue). Exposure time was the same in all cases. Bar, 20 μm.
