Figure 4.

JAK-STAT inhibition partially rescues hyperactive inflammatory responses in C9orf72^−/− RAW264.7 cells

(A-F) Control or C9orf72^−/− RAW264.7 cells were treated with or without ruxolitinib (1 μM) for 16hrs and then treated with DMXAA (A-C) or IFNα (1000 U/mL) (D-F) for additional 16hrs with or without ruxolitinib. Cxcl10, Stat1, and Sting mRNA levels were assayed by RT-qPCR. Data represent the mean ± SEM. Statistical significance was analyzed by two-way ANOVA (n = 3). Groups that exhibit non-significant differences with two-way ANOVA were re-analyzed using unpaired two-tailed Student’s t test, and the results are indicated in parentheses. ns = not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.