The architectonic factor CTCF. (a) CTCF is an 82-kDa protein that contains three domains: an N-terminal region, a C-terminal region, and a central domain of 11 zinc fingers. Moreover, CTCF uses the zinc finger domain cooperatively to bind to DNA. RBD:RNA binding domain, ZF: zinc finger. (b) Overview of the wide arrange of CTCF mechanisms of action as: Chromatin looping, RNA Polymerase II (Pol II) recruitment, transcriptional regulation, boundary definition, DNA anchorage, insulator, alternative splicing, and RNA binding, among others. TAD: topologically associated domain. Created with BioRender.com (accessed on 23 April 2023).