Table 1.
Comparison of fatty acid methyl ester (FAME) percentages (% rel. quant.) obtained from the same RBC membrane pellet transformed by alkaline (0.5 M KOH/MeOH; room temperature) and by acidic (0.5 M HCl/MeOH—100 °C) transesterification conditions (n = 10 repetitions each).
| FAME 1 | KOH/MeOH (% Rel. Quant.) Mean ± SD (n = 10) |
HCl/MeOH (% Rel. Quant.) Mean ± SD (n = 10) 2 |
|---|---|---|
| 16:0 | 23.3 ± 0.6 | 34.0 ± 3.2 *** |
| 9cis-16:1 | 0.24 ± 0.03 | 0.18 ± 0.05 ** |
| 18:0 | 16.0 ± 1.3 | 21.8 ± 1.3 *** |
| 9trans-18:1 | 0.01 ± 0.01 | 0.03 ± 0.01 *** |
| 9cis-18:1 | 17.0 ± 0.5 | 13.8 ± 1.0 *** |
| 11cis-18:1 | 1.2 ± 0.2 | 1.0 ± 0.1 *** |
| 9cis,12cis-18:2 | 12.1±0.6 | 9.2 ± 0.4 *** |
| 20:3 omega-6, DGLA | 2.3 ± 0.1 | 1.5 ± 0.3 *** |
| 20:4 omega-6, Ara | 18.8 ± 0.4 | 13.3 ± 1.4 *** |
| 5mtAra | 0.02 ± 0.01 | 0.03 ± 0.01 * |
| 20:5 omega-3, EPA | 0.8 ± 0.1 | 0.6 ± 0.3 * |
| 22:5 omega-3, DPA | 2.7 ± 0.5 | 1.7 ± 0.4 *** |
| 22:6 omega-3, DHA | 5.4 ± 0.1 | 3.0 ± 0.5 *** |
| SFA | 39.3 ± 1.3 | 55.8 ± 3.3 *** |
| MUFA | 18.5 ± 0.7 | 14.9 ± 0.9 *** |
| PUFA | 42.1 ± 0.7 | 29.3 ± 2.6 *** |
| omega-6 | 33.2 ± 0.7 | 24.0 ± 1.8 *** |
| omega-3 | 8.9 ± 0.4 | 5.2 ± 0.9 *** |
| TOT TRANS | 0.03 ± 0.01 | 0.05 ± 0.02 * |
1 Identified by standard references and quantified as described in Materials and Methods. Values are obtained in µg/µL from the GC peak areas recognized and calibrated with standard references (corresponding to >97% of the total peaks of the chromatogram). Values are expressed in percentages relative to the sum of all the quantities of the recognized peaks (100%) ± Standard Deviation (S.D); 2 Statistical significance is estimated: * p < 0.043; ** p ≤ 0.0044; *** p ≤ 0.0005.