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. 2023 May 13;12(10):1380. doi: 10.3390/cells12101380

Figure 2.

Figure 2

Nuclear envelope permeabilization and centrosome duplication occur at the onset of mitosis. Selected time points of Videos S3 and S4 are shown. (A) NE marker Src1-Neon knock-in (green) with the NE permeabilization marker NLS-TdTom (magenta). The nuclear envelope is preserved during the entire cell cycle, while NLS-TdTom diffuses into the cytosol (arrow at 100 s) and is restored long after cytokinesis (920 s). (B) Cell with Src1-Neon knock-in (green) and centrosomal component CP75-Ruby knock-in (magenta). The centrosome moves from a perinuclear position (−67.2 s) into the nuclear envelope (−33.6 s) before it duplicates (arrowhead at 0 s). Bars 5 µm.