Figure 3.

arg8∆ and arg2∆/arg7∆ mutants are auxotrophic for arginine. (A,C–F), Growth behavior of arg8∆ and arg2∆/arg7∆ mutants grown in SC medium in the presence or absence of arginine and glucose as sole carbon source. (A,C–E), Ten-fold serial dilutions of wild-type BY4742 cells and various yeast strains were plated. (F), The various yeast strains were inoculated in SC medium supplemented with 2% glucose in the presence or absence of arginine. The values of optical density at 600 nm refer to cell cultures after the indicated periods of growth. Data from three technical replicates are reported. Similar results were obtained in three independent experiments. (B), Expression levels of argA-E in the arg8∆ expressing argB-E or argA-E assayed with an anti-HA and anti-Myc tags specific antibodies (see text for more details). The loaded extracts were normalized by an anti-Aac2p antibody.